FUS2 Antibody (2F4) - BSA Free

Western Blot: FUS2 Antibody (2F4) - BSA Free [NBP1-41157] - Cell lysates of NIH3T3(40ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human FUS2 (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Immunocytochemistry/Immunofluorescence: FUS2 Antibody (2F4) - BSA Free [NBP1-41157] - Immunofluorescence of human A549 cells stained with Hoechst 33342 (Blue) and monoclonal anti-human FUS2 antibody (1:500) with Texas Red (red).

Flow Cytometry: FUS2 Antibody (2F4) - BSA Free [NBP1-41157] - Flow cytometry analysis of FUS2 in Balb/3T3 cells. The cell was stained with NBP1-41157 at 2-5ug for 1x10^6cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).

• Reactivity: Hu
• Applications: WB, ELISA, Flow, ICC/IF
• Clone: 2F4
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

FUS2 Antibody (2F4) - BSA Free Summary

• Immunogen: Recombinant FUS2 (1-308aa) purified from E. coli
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: NAA80
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence 1:500-1:1000
  • Western Blot 1:500-1:1000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4), 10% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein G purified

Alternate Names for FUS2 Antibody (2F4) - BSA Free

• FUS-2
• FUS2EC 2.3.1.-
• N-acetyltransferase 6 (GCN5-related)
• N-acetyltransferase 6
• Protein fus-2
• Protein fusion-2

Background

Vertebrate FUS2 genes, which are known to be putative tumor suppressor gene, contain several important domains such as the catalytic N-acetyltransferase (NAT) domain. NAT domain is essential enzymes involved in several sophisticated cellular processes such as N-acetylation, O-acetylatin. NAT enzymes may be involved in susceptibility to cancer including colorectal cancer because of the presence of carcinogenic heterocyclic amines in some cooked foods. FUS2 was physically localized to the cytoplasm. Also, FUS2 showed the actin dependent movement, closely related to the polarization in the budding yeast, Saccharomyces cerevisiae.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NAA80
• Entrez:
  • 24142(Human)
• Uniprot:
  • NP_036323(Human)