FUBI/MNSF beta/FAU Overexpression Lysate

Images

 
Western Blot: FAU Overexpression Lysate (Adult Normal) [NBL1-10606] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for FAU.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

FUBI/MNSF beta/FAU Overexpression Lysate Summary

Description

FUBI/MNSF beta/FAU Transient Overexpression Lysate


Expression Host: HEK293T

Plasmid: RC207711

Accession#: NM_001997

Protein Tag: C-MYC/DDK
Gene
FAU

Applications/Dilutions

Dilutions
  • Western Blot
Application Notes
This product is intended for use as a positive control in Western Blot.

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul).
Theoretical MW
14.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Array

Type
Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for FUBI/MNSF beta/FAU Overexpression Lysate

  • 40S Ribosomal Protein S30
  • asr1
  • FAU
  • FAU1
  • FAU-encoded ubiquitin-like protein
  • FBR-MuSV-associated ubiquitously expressed
  • Finkel-Biskis-Reilly murine sarcoma virus (FBR-MuSV) ubiquitously expressed
  • Finkel-Biskis-Reilly murine sarcoma virus (FBR-MuSV) ubiquitously expressed(fox derived)
  • FLJ22986
  • Fub1
  • FUBI
  • MNSFbeta
  • monoclonal nonspecific suppressor factor beta
  • ribosomal protein S30
  • RPS30
  • S30
  • ubiquitin-like protein fubi and ribosomal protein S30
  • ubiquitin-like-S30 fusion protein

Background

This gene is the cellular homolog of the fox sequence in the Finkel-Biskis-Reilly murine sarcoma virus (FBR-MuSV). It encodes a fusion protein consisting of the ubiquitin-like protein fubi at the N terminus and ribosomal protein S30 at the C terminus. It has been proposed that the fusion protein is post-translationally processed to generate free fubi and free ribosomal protein S30. Fubi is a member of the ubiquitin family, and ribosomal protein S30 belongs to the S30E family of ribosomal proteins. Whereas the function of fubi is currently unknown, ribosomal protein S30 is a component of the 40S subunit of the cytoplasmic ribosome. Pseudogenes derived from this gene are present in the genome. Similar to ribosomal protein S30, ribosomal proteins S27a and L40 are synthesized as fusion proteins with ubiquitin. [provided by RefSeq]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Publications for FUBI/MNSF beta/FAU Lysate (NBL1-10606) (0)

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Bioinformatics

Gene Symbol FAU