Recombinant Human Frequenin His Protein

SDS-Page: Frequenin Protein [NBP1-44484] - NCS1, 22.9 kDa (198aa), confirmed by MALDI-TOF with a purity of 95% by SDS - PAGE

• Reactivity: Hu
• Applications: PAGE
• Concentration: 1 mg/ml

Recombinant Human Frequenin His Protein Summary

• Description: A recombinant protein with a C-Terminal His-tag and corresponding to the amino acids 1- 190 of Human Frequenin
  
  

  Source: E.coli

  Amino Acid Sequence: MGKSNSKLKP EVVEELTRKT YFTEKEVQQW YKGFIKDCPS GQLDAAGFQK IYKQFFPFGD PTKFATFVFN VFDENKDGRI EFSEFIQALS VTSRGTLDEK LRWAFKLYDL DNDGYITRNE MLDIVDAIYQ MVGNTVELPE EENTPEKRVD RIFAMMDKNA DGKLTLQEFQ EGSKADPSIV QALSLYDGLV LEHHHHHH

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: NCS1
• Purity: >95%, by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • SDS-Page
• Theoretical MW: 22.9 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20 mM Tris-HCl buffer (pH 8.0), 1 mM DTT, 10% glycerol
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: >95%, by SDS-PAGE

Alternate Names for Recombinant Human Frequenin His Protein

• DKFZp761L1223
• FREQFLUP
• frequenin (Drosophila) homolog
• frequenin homolog (Drosophila)
• Frequenin homolog
• Frequenin-like protein
• Frequenin-like ubiquitous protein
• NCS-1
• neuronal calcium sensor 1

Background

Neuronal calcium sensor 1, also known as NCS1, is a member of the neuronal calcium sensor gene family, which encode calcium-binding proteins expressed predominantly in neurons. NCS1 is localized to neuronal cell bodies and axons throughout the brain and spinal cord. It is also expressed in glial cells and in neuroendocrine bovine adrenal chromaffin and PC12 cells. NCS1 is a regulatory protein involved in Ca++-dependent exocytosis of synaptic vesicles and dense core granules. Recombinant human NCS1 fused to His-tag at C-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: NCS1