Recombinant Human Frequenin Protein

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SDS-Page: Frequenin Protein [NBP1-44484] - NCS1, 22.9 kDa (198aa), confirmed by MALDI-TOF with a purity of 95% by SDS - PAGE

Product Details

Summary
Reactivity HuSpecies Glossary
Applications PAGE
Concentration
1.0 mg/ml

Order Details

Recombinant Human Frequenin Protein Summary

Description
A recombinant protein with a His tag and corresponding to amino acids 1 - 190 of NCS1.

Source: E. coli

Amino Acid Sequence: MGKSNSKLKP EVVEELTRKT YFTEKEVQQW YKGFIKDCPS GQLDAAGFQK IYKQFFPFGD PTKFATFVFN VFDENKDGRI EFSEFIQALS VTSRGTLDEK LRWAFKLYDL DNDGYITRNE MLDIVDAIYQ MVGNTVELPE EENTPEKRVD RIFAMMDKNA DGKLTLQEFQ EGSKADPSIV QALSLYDGLV LEHHHHHH

Preparation
Method
E.coli
Protein/Peptide Type
Recombinant Protein
Gene
NCS1
Purity
>95% pure by SDS-PAGE

Applications/Dilutions

Theoretical MW
22.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

This is a Human protein

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol
Preservative
No Preservative
Concentration
1.0 mg/ml
Purity
>95% pure by SDS-PAGE

Notes

The purity of this protein is > 95% by SDS-PAGE. Molecular weight is 22.9 kDa (198aa), confirmed by MALDI-TOF

Alternate Names for Recombinant Human Frequenin Protein

  • DKFZp761L1223
  • FREQFLUP
  • frequenin (Drosophila) homolog
  • frequenin homolog (Drosophila)
  • Frequenin homolog
  • Frequenin-like protein
  • Frequenin-like ubiquitous protein
  • NCS-1
  • neuronal calcium sensor 1

Background

Neuronal calcium sensor 1, also known as NCS1, is a member of the neuronal calcium sensor gene family, which encode calcium-binding proteins expressed predominantly in neurons. NCS1 is localized to neuronal cell bodies and axons throughout the brain and spinal cord. It is also expressed in glial cells and in neuroendocrine bovine adrenal chromaffin and PC12 cells. NCS1 is a regulatory protein involved in Ca++-dependent exocytosis of synaptic vesicles and dense core granules. Recombinant human NCS1 fused to His-tag at C-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Bioinformatics

Gene Symbol NCS1

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