FPRL2/FPR3 Antibody

• Reactivity: Hu
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

FPRL2/FPR3 Antibody Summary

• Description: Please note, this antibody is considered to be "Innovators Grade". Innovators Grade antibodies have been validated for limited uses and require additional characterization for most new species/applications. These antibodies are directed against antigens that have limited or no commercial antibodies available for them, or for which there are limited expression data available to us. In an effort to encourage customer feedback, Novus has made these antibodies available through our Innovators Reward program. Under this program, Novus will provide you a 50% refund on the purchased product as well as a 50% discount on a future product of equal value in exchange for your new data. Please contact us at innovators@novusbio.com for more details.
• Immunogen: Peptide with sequence C-DSAQTSNTDTTS corresponding to C-Terminus according to NP_002021.3.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: FPR3
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:1000
  • Western Blot 1:100 - 1:2000
• Application Notes: WB: Preliminary experiments gave an approx. 55 kDa band in human lymph node lysates after 0.3 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 40.0 kDa band according to NP_002021.3. However, we do note that the protein is a glycoprotein. The 55 kDa band was successfully blocked by incubation with the immunizing peptide.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for FPRL2/FPR3 Antibody

• FMLP-R-II
• FMLPY
• formyl peptide receptor 3
• Formyl peptide receptor-like 2FMLP-related receptor II
• FPR3
• FPRH1
• FPRH1FPRH2
• FPRH2
• FPRL2
• FPRL2FML2_HUMAN
• N-formyl peptide receptor 3
• RMLP-R-I

Background

FPRL2 is a Chemoattractant Receptor involved in the transduction of signals in myeloid cells. Although it is structurally homologous to FPRL1, human FPRL2 does not recognize the N-formylmethionyl peptides (FMLP) as ligands and is not expressed in neutrophils.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for FPRL2/FPR3 Antibody (NBP2-26058)(1)

Publication using NBP2-26058

• Gao JL, Guillabert A, Hu J et al. F2L, a peptide derived from heme-binding protein, chemoattracts mouse neutrophils by specifically activating Fpr2, the low-affinity N-formylpeptide receptor. J Immunol 2007-02-01 [PMID: 17237393]

Bioinformatics

• Gene Symbol: FPR3
• Entrez:
  • 2359(Human)
  • 2359(Human)