FNBP4 Antibody - BSA Free

Immunoprecipitation: FNBP4 Antibody [NB100-74644] - Detection of Human FNBP4/FBP30 by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-FNBP4/FBP30 antibody NB100-74644 used for IP at 3 mcg/mg lysate. FNBP4/FBP30 was also immunoprecipitated by rabbit anti-FNBP4/FBP30 antibody NB100-74645, which recognizes a downstream epitope. Detection: Chemiluminescence with an exposure time of 10 seconds.

Product Discontinued

FNBP4 Antibody - BSA Free Summary

• Immunogen: The immunogen recognized by this antibody maps to a region between residue 1 and 50 of human formin binding protein 4 (formin-binding protein 30) using the numbering given in entry EAW67887.1 (GeneID 23360).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: FNBP4
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 2-5ug/mg Lysate
• Application Notes: Not recommended for western blot.
• Control:

  FNBP4 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for FNBP4 Antibody - BSA Free

• DKFZp686M14102
• FBP30
• FLJ41904
• formin binding protein 4
• Formin-binding protein 30
• formin-binding protein 4
• KIAA1014DKFZp779I1064

Background

Partial sequence of FNBP4/FBP30 was originally isolated in a screen for proteins that interact with formins, proteins involved in murine limb and kidney development. The full length FNBP4/FBP30 was identified in a screen for genes upregulated in irradiated thymocytes. FNBP4/FBP30 bears two WW domains, a domain that resembles the src-homology 3 (SH3) domain and is known to associate with proline-rich motifs.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: FNBP4
• Entrez:
  • 23360(Human)
• Uniprot:
  • EAW67887.1(Human)