Flamingo homolog 1/CELSR3 Antibody [CoraFluor™ 1]

CoraFluor™ 1, amine reactive (Catalog:7920) and CoraFluor™ 2, amine reactive (Catalog # 7950) are terbium-based probes that have been developed for use as TR-FRET donors. They emit wavelengths compatible with commonly used fluorescent acceptor dyes such as BODIPY® (or BDY) and Janelia Fluor® dyes, FITC (Catalog # 5440), TMR and Cyanine 5 (Catalog # 5436). CoraFluor™ fluorescence is brighter and more stable in biological media than existing TR-FRET donors, leading to enhanced sensitivity and improved data generation. CoraFluor™ 1 exhibits excitation upon exposure to a 337 nm UV laser.

• Reactivity: Hu
• Applications: Flow, CyTOF-ready
• Clonality: Polyclonal
• Host: Goat
• Conjugate: CoraFluor 1

Flamingo homolog 1/CELSR3 Antibody [CoraFluor™ 1] Summary

• Description: CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(TM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.
  
  CoraFluor(TM) 1, amine reactive
  
  CoraFluor(TM) 1, thiol reactive
  
  For more information, please see our CoraFluor(TM) TR-FRET technology flyer.
• Immunogen: E. coli-derived recombinant human CELSR3
  Tyr531-Ser711
  Accession # Q9NYQ7
• Specificity: Detects human CELSR3 in direct ELISAs. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) CELSR1 and rhCELSR2 is observed.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: CELSR3
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark. Do not freeze.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Antigen Affinity-purified

Notes

CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254

Alternate Names for Flamingo homolog 1/CELSR3 Antibody [CoraFluor™ 1]

• Cadherin family member 11
• cadherin, EGF LAG seven-pass G-type receptor 3 (flamingo homolog, Drosophila)
• CDHF11
• CDHF11RESDA1
• CELSR3
• EGFL1
• EGFL1cadherin EGF LAG seven-pass G-type receptor 3
• EGF-like protein 1
• EGF-like-domain, multiple 1
• epidermal growth factor-like 1
• Epidermal growth factor-like protein 1
• Flamingo homolog 1
• FMI1anchor protein
• HFMI1
• KIAA0812
• MEGF2
• MEGF2cadherin EGF LAG seven-pass G-type receptor 3, flamingo (Drosophila) homolog
• multiple EGF-like domains 2
• Multiple EGF-like domains protein 2
• Multiple epidermal growth factor-like domains protein 2
• RESDA1

Background

CELSR3 is an Orphan-U GPCR with an unknown ligand. In mouse, this gene is expressed during development at sites of active neurogenesis and in adult mice and rats in brain, spinal cord, dorsal root ganglion, and eye. ESTs have been isolated from human B-cell/lung/testis, blood, brain, colon, heart/melanocyte/uterus, lung, nerve, and placenta libraries.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CELSR3