FHIT Antibody

Product Discontinued

FHIT Antibody Summary

• Immunogen: Recombinant full length protein (Human).
• Localization: Cytoplasmic
• Specificity: FHIT
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: FHIT
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:200
• Application Notes: Immunohistochemistry-Paraffin Perform enzymatic antigen retrieval before commencing with IHC staining protocol, by boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.

Reactivity Notes

Cross-reacts with Human. Not yet tested in other species.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 10mM PBS (pH 7.4) and BSA
• Preservative: Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for FHIT Antibody

• AP3A hydrolase
• AP3AaseFRA3Bbis(5'-adenosyl)-triphosphatase
• Diadenosine 5'-5'''-P1
• dinucleosidetriphosphatase
• EC 3.6.1.29
• fragile histidine triad gene
• Fragile histidine triad protein
• P3-triphosphate hydrolase
• tumor suppressor protein

Background

FHIT (fragile histidine triad) cleaves adenosine 5' PPP 5' A to yield AMP and ADP. Alterations and deletions of the FHIT gene are strongly linked to the genesis and establishment of human tumors of the lung, cervix, breast, colon, stomach and pancreas. In normal cells, FHIT may act as a tumor suppressor. FHIT physically associates with ubiquitin conjugating enzyme 9.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: FHIT
• Entrez:
  • 2272(Human)