FFAR1/GPR40 Antibody (1048036) [Unconjugated]

FFAR1/GPR40 was detected in immersion fixed paraffin-embedded sections of human brain hypothalamus using Mouse Anti-Human FFAR1/GPR40 Monoclonal Antibody (Catalog # MAB11025) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in neurons. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

• Reactivity: Hu
• Applications: IHC
• Clone: 1048036
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

FFAR1/GPR40 Antibody (1048036) [Unconjugated] Summary

• Immunogen: Synthetic peptide within aminoacids 1-50 of human FFAR1/GPR40
  
  Accession # O14842
• Specificity: Detects human FFAR1/GPR40 in ELISA.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 3-25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for FFAR1/GPR40 Antibody (1048036) [Unconjugated]

• FFA1
• FFAR1
• free fatty acid receptor 1
• G protein-coupled receptor 40
• GPCR40
• GPR40
• GPR40FFA1R
• G-protein coupled receptor 40

Background

Human Free Fatty Acid Receptor 1 (FFAR-1), also known as GPR40, is a class A G-protein coupled receptor (GPCR) for medium and long chain saturated and unsaturated fatty acids encoded by the FFAR1 gene. FFAR-1 is expressed in pancreas and it plays an important role in glucose homeostasis acting as a nutrient sensor. Fatty acid binding increases glucose-stimulated insulin secretion, and may also enhance the secretion of glucagon-like peptide 1 (GLP-1).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • O14842(Human)