FATP4/SLC27A4 Knockout 293T Cell Lysate

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB
Concentration
LYOPH

Order Details

FATP4/SLC27A4 Knockout 293T Cell Lysate Summary

Preparation
Method
Knockout achieved by using CRISPR/Cas9,14 bp deletion in exon 2
Gene
SLC27A4

Applications/Dilutions

Application Notes
You will receive 1 vial (100ug) of knockout cell lysate and 1 vial (100ug) of Parental cell lysate. Lysate can be diluted with 1X SDS sample buffer and will be stable at -20 degrees C for 12 months. Minimize freeze-thaw cycles.

Packaging, Storage & Formulations

Storage
Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.
Buffer
0.1 mg cell homogenate lyophilized in RIPA buffer made with double-knockout cell lines.
Concentration
LYOPH
Reconstitution Instructions
To use as WB negative control, spin down briefly and resuspend in 100 uL 1xSDS sample buffer (2% SDS, 60 mM Tris-HCl pH 6.8, 10% Glycerol, 0.02% Bromophenol blue, 60 mM beta-mercaptoethanol). Boil the lysate for 3 - 5 minutes before loading it onto gel.

Lysate Details for Array

Type
Knockout 293T Cell
Life Stage
Embryonic
Tissue
Kidney

Notes

Powered by EDIGENE.
Validation of antibody specificity is critical and verification of antibody performance against knockout samples is one way to guarantee that an antibody recognizes a specific target. Novus' KO cell lysate can be used as a negative control for western blots and to confirm the specificity of antibodies.

Alternate Names for FATP4/SLC27A4 Knockout 293T Cell Lysate

  • ACSVL4
  • ACSVL4Solute carrier family 27 member 4
  • EC 6.2.1
  • EC 6.2.1.-
  • EC 6.2.1.7
  • FATP4
  • FATP-4
  • FATP4Fatty acid transport protein 4
  • IPS
  • long-chain fatty acid transport protein 4
  • SLC27A4
  • solute carrier family 27 (fatty acid transporter), member 4

Background

This gene encodes a member of a family of fatty acid transport proteins, which are involved in translocation of long-chain fatty acids cross the plasma membrane. This protein is expressed at high levels on the apical side of mature enterocytes in the small intestine, and appears to be the principal fatty acid transporter in enterocytes. Clinical studies suggest this gene as a candidate gene for the insulin resistance syndrome. Mutations in this gene have been associated with ichthyosis prematurity syndrome. [provided by RefSeq, Apr 2010]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Other Available Formats

NBP2-66404

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Bioinformatics

Gene Symbol SLC27A4