Recombinant Human Eukaryotic translation initiation factor 1 Protein

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SDS-Page: Eukaryotic translation initiation factor 1 Protein [NBP1-37080] - EIF1, 16.9 kDa (150aa), confirmed by MALDI-TOF with a purity of 90% by SDS - PAGE

Product Details

Summary
Reactivity HuSpecies Glossary
Applications PAGE
Concentration
0.5 mg/ml

Order Details

Recombinant Human Eukaryotic translation initiation factor 1 Protein Summary

Description
A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-113 of Human Eukaryotic translation initiation factor 1

Source: E.coli

Amino Acid Sequence: MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWAGSMSA IQNLHSFDPF ADASKGDDLL PAGTEDYIHI RIQQRNGRKT LTTVQGIADD YDKKKLVKAF KKKFACNGTV IEHPEYGEVI QLQGDQRKNI CQFLVEIGLA KDDQLKVHGF

Source
E. coli
Protein/Peptide Type
Recombinant Protein
Gene
EIF1
Purity
>90%, by SDS-PAGE

Applications/Dilutions

Theoretical MW
16.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
20 mM Tris-HCl buffer (pH8.0) containing 1mM DTT, 0.1 M NaCl, and 10% glycerol
Preservative
No Preservative
Concentration
0.5 mg/ml
Purity
>90%, by SDS-PAGE

Alternate Names for Recombinant Human Eukaryotic translation initiation factor 1 Protein

  • A121Protein translation factor SUI1 homolog
  • eIF1
  • EIF-1
  • EIF1A
  • eukaryotic translation initiation factor 1
  • ISO1
  • Sui1iso1
  • SUI1sui1iso1

Background

EIF1, also known as eukaryotic translation initiation factor 1, is a universally conserved translation factor that is necessary for scanning and involved in initiation site selection. This protein promotes the assembly of 48S ribosomal complexes at the authentic initiation codon of a conventional capped mRNA. Recombinant human EIF1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Bioinformatics

Gene Symbol EIF1