ENTPD8 Antibody

Immunohistochemistry-Paraffin: ENTPD8 Antibody [NBP1-82517] - Staining in human small intestine and pancreas tissues using anti-ENTPD8 antibody. Corresponding ENTPD8 RNA-seq data are presented for the same tissues.

Immunocytochemistry/Immunofluorescence: ENTPD8 Antibody [NBP1-82517] - Staining of human cell line A-431 shows localization to nucleoplasm & cytosol. Antibody staining is shown in green.

Immunohistochemistry-Paraffin: ENTPD8 Antibody [NBP1-82517] - Staining of human small intestine shows high expression.

Immunohistochemistry-Paraffin: ENTPD8 Antibody [NBP1-82517] - Staining of human pancreas shows low expression as expected.

• Reactivity: Hu
• Applications: ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

ENTPD8 Antibody Summary

• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: SHTSLFLYQWPANKENGTGVVSQALACQVEGPGISSYTSNAAQAGESLQGCLEEALVLIPEAQHRKTPTFLGATAGMRLLSRKNSSQARDIFAAVTQVLGRSPVDFWGAELLAGQAEGAFGWITVNYGLG
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ENTPD8
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
• Control Peptide:

  ENTPD8 Protein (NBP1-82517PEP)

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (80%), Rat (81%)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for ENTPD8 Antibody

• EC 3.6.1
• EC 3.6.1.5
• ecto-ATP-diphosphohydrolase
• ectonucleoside triphosphate diphosphohydrolase 8
• E-NTPDase 8
• GLSR2492
• liver ecto-ATP-diphosphohydrolase
• NTPDase 8
• NTPDase8
• NTPDase-8
• nucleoside triphosphate diphosphohydrolase-8
• UNQ2492

Background

Canalicular ectonucleoside NTPDase responsible for the main hepatic NTPDase activity. Ectonucleoside NTPDasescatalyze the hydrolyzis of gamma- and beta-phosphate residues of nucleotides, playing a central role in concentrationof extracellular nucleotides. Has activity toward ATP, ADP, UTP and UDP, but not toward AMP

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ENTPD8