EIF5 Antibody (2E6-4C12) - Azide and BSA Free

Western Blot: EIF5 Antibody (2E6-4C12) [H00001983-M01] - Analysis of EIF5 expression in transfected 293T cell line by EIF5 monoclonal antibody (M01), clone 2E6-4C12.Lane 1: EIF5 transfected lysate(49.2 KDa).Lane 2: Non-transfected lysate.

Immunocytochemistry/Immunofluorescence: EIF5 Antibody (2E6-4C12) [H00001983-M01] - Analysis of monoclonal antibody to EIF5 on HeLa cell. Antibody concentration 10 ug/ml.

Immunohistochemistry-Paraffin: EIF5 Antibody (2E6-4C12) [H00001983-M01] - Analysis of monoclonal antibody to EIF5 on formalin-fixed paraffin-embedded human malignant lymphoma, diffuse large B tissue. Antibody concentration 1 ug/ml.

Western Blot: EIF5 Antibody (2E6-4C12) [H00001983-M01] - EIF5 monoclonal antibody (M01), clone 2E6-4C12 Analysis of EIF5 expression in Jurkat.

Sandwich ELISA: EIF5 Antibody (2E6-4C12) [H00001983-M01] - Detection limit for recombinant GST tagged EIF5 is approximately 1ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF, IHC
• Clone: 2E6-4C12
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

EIF5 Antibody (2E6-4C12) - Azide and BSA Free Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: EIF5 (AAH32866, 1 a.a. ~ 431 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MSVNVNRSVSDQFYRYKMPRLIAKVEGKGNGIKTVIVNMVDVAKALNRPPTYPTKYFGCELGAQTQFDVKNDRYIVNGSHEANKLQDMLDGFIKKFVLCPECENPETDLHVNPKKQTIGNSCKACGYRGMLDTHHKLCTFILKNPPENSDSGTGKKEKEKKNRKGKDKENGSVSSSETPPPPPPPNEINPPPHTMEEEEDDDWGEDTTEEAQRRRMDEISDHAKVLTLSDDLERTIEERVNILFDFVKKKKEEGVIDSSDKEIVAEAERLDVKAMGPLVLTEVLFNEKIREQIKKYRRHFLRFCHNNKKAKRYLLHGLECVVAMHQAQLISKIPHILKEMYDADLLEEEVIISWSEKASKKYVSKELAKEIRVKAEPFIKWLKEAEEESSGGEEEDEDENIEVVYSKAASVPKVETVKSDNKDDDIDIDAI
• Specificity: EIF5 - eukaryotic translation initiation factor 5
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: EIF5
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Sandwich ELISA
  • Western Blot 1:500
• Application Notes: Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IF, IHC-P and ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for EIF5 Antibody (2E6-4C12) - Azide and BSA Free

• eIF-5
• EIF-5A
• eukaryotic translation initiation factor 5

Background

EIF5 catalyzes the hydrolysis of sphingomyelin to form ceramide and phosphocholine. Ceramide mediates numerous cellular functions, such as apoptosis and growth arrest, and is capable of regulating these 2 cellular events independently. Also hydrolyzes sphingos

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: EIF5
• Entrez:
  • 1983(Human)
• OMIM:
  • 601710(Human)
• Uniprot:
  • AAH32866(Human)