EEF2K Antibody

Western Blot: EEF2K Antibody [NBP3-03721] - Western blot analysis of extracts of various cell lines, using EEF2K antibody (NBP3-03721) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 40s.

Immunocytochemistry/Immunofluorescence: EEF2K Antibody [NBP3-03721] - Immunofluorescence analysis of A-549 cells using EEF2K antibody (NBP3-03721). Blue: DAPI for nuclear staining.

Knockout Validated: EEF2K Antibody [NBP3-03721] - Western blot analysis of extracts from normal (control) and EEF2K knockout (KO) HeLa cells, using EEF2K antibody (NBP3-03721) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Product Discontinued

EEF2K Antibody Summary

• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 406-725 of human EEF2K (NP_037434.1).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: EEF2K
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50 - 1:200
  • Knockout Validated
  • Western Blot 1:500 - 1:2000
• Theoretical MW: 82 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS with 50% glycerol, pH7.3.
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for EEF2K Antibody

• Calcium/calmodulin-dependent eukaryotic elongation factor 2 kinase
• calcium/calmodulin-dependent eukaryotic elongation factor-2 kinase
• EC 2.7.11
• EC 2.7.11.20
• eEF-2 kinase
• eEF-2Kcalmodulin-dependent protein kinase III
• elongation factor-2 kinase
• eukaroytic elongation factor 2 kinase
• eukaryotic elongation factor 2 kinase
• eukaryotic elongation factor-2 kinase
• HSU93850
• MGC45041

Background

EEF2K encodes a highly conserved protein kinase in the calmodulin-mediated signaling pathway that links activation of cell surface receptors to cell division. This kinase is involved in the regulation of protein synthesis. It phosphorylates eukaryotic elongation factor 2 (EEF2) and thus inhibits the EEF2 function. The activity of this kinase is increased in many cancers and may be a valid target for anti-cancer treatment. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: EEF2K