Recombinant Human EEF1D Protein

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SDS-Page: Recombinant Human EEF1D Protein [NBP1-51028] - 15 % SDS-PAGE (3ug)

Product Details

Summary
Reactivity HuSpecies Glossary
Applications PAGE
Concentration
0.5 mg/ml

Order Details

Recombinant Human EEF1D Protein Summary

Description
A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-281 of Human EEF1D

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MATNFLAHEK IWFDKFKYDD AERRFYEQMN GPVAGASRQE NGASVILRDI ARARENIQKS LAGSSGPGAS SGTSGDHGEL VVRIASLEVE NQSLRGVVQE LQQAISKLEA RLNVLEKSSP GHRATAPQTQ HVSPMRQVEP PAKKPATPAE DDEDDDIDLF GSDNEEEDKE AAQLREERLR QYAEKKAKKP ALVAKSSILL DVKPWDDETD MAQLEACVRS IQLDGLVWGA SKLVPVGYGI RKLQIQCVVE DDKVGTDLLE EEITKFEEHV QSVDIAAFNK I

Source
E. coli
Protein/Peptide Type
Recombinant Protein
Gene
EEF1D
Purity
>90%, by SDS-PAGE

Applications/Dilutions

Theoretical MW
33.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol
Preservative
No Preservative
Concentration
0.5 mg/ml
Purity
>90%, by SDS-PAGE

Alternate Names for Recombinant Human EEF1D Protein

  • Antigen NY-CO-4
  • EF-1D
  • EF-1-delta
  • EF1DFP1047
  • elongation factor 1-delta
  • eukaryotic translation elongation factor 1 delta (guanine nucleotide exchangeprotein)
  • FLJ20897
  • guanine nucleotide exchange protein

Background

Eukaryotic translation elongation factor 1 delta, also known as EEF1D, is a subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. This subunit functions as guanine nucleotide exchange factor. It is reported that this subunit interacts with HIV-1 Tat, and thus it represses the translation of host-cell, but not HIV-1, mRNAs. Overexpression of EEF1D is associated with oesophageal carcinoma and may adversely affect the outcome of medulloblastomas. Recombinant human EEF1D protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Bioinformatics

Gene Symbol EEF1D