EEF1D Antibody

Western Blot: EEF1D Antibody [H00001936-B01P] - Analysis of EEF1D expression in human pancreas.

Immunocytochemistry/Immunofluorescence: EEF1D Antibody [H00001936-B01P] - Analysis of purified antibody to EEF1D on HeLa cell. (antibody concentration 10 ug/ml)

Western Blot: EEF1D Antibody [H00001936-B01P] - Analysis of EEF1D expression in HeLa.

Western Blot: EEF1D Antibody [H00001936-B01P] - Analysis of EEF1D expression in transfected 293T cell line by EEF1D polyclonal antibody. Lane 1: EEF1D transfected lysate(30.91 KDa). Lane 2: Non-transfected lysate.

Product Discontinued

EEF1D Antibody Summary

• Description: Quality control test: Antibody reactive against mammalian transfected lysate.
• Immunogen: EEF1D (NP_001951.2, 1 a.a. - 281 a.a.) full-length human protein. MATNFLAHEKIWFDKFKYDDAERRFYEQMNGPVAGASRQENGASVILRDIARARENIQKSLAGSSGPGASSGTSGDHGELVVRIASLEVENQSLRGVVQELQQAISKLEARLNVLEKSSPGHRATAPQTQHVSPMRQVEPPAKKPATPAEDDEDDDIDLFGSDNEEEDKEAAQLREERLRQYAEKKAKKPALVAKSSILLDVKPWDDETDMAQLEACVRSIQLDGLVWGASKLVPVGYGIRKLQIQCVVEDDKVGTDLLEEEITKFEEHVQSVDIAAFNKI
• Specificity: EEF1D - eukaryotic translation elongation factor 1 delta (guanine nucleotide exchange protein),
• Isotype: IgG
• Clonality: Polyclonal
• Host: Mouse
• Gene: EEF1D
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot
• Application Notes: Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB and also on transfected lysate in WB. GST tag alone is used as a negative control. It is also useful for IF.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4)
• Preservative: No Preservative
• Purity: Protein A purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for EEF1D Antibody

• Antigen NY-CO-4
• EF-1D
• EF-1-delta
• EF1DFP1047
• elongation factor 1-delta
• eukaryotic translation elongation factor 1 delta (guanine nucleotide exchangeprotein)
• FLJ20897
• guanine nucleotide exchange protein

Background

This gene encodes a subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. This subunit functions as guanine nucleotide exchange factor. It is reported that this subunit interacts with HIV-1 Tat, and thus it represses the translation of host-cell, but not HIV-1, mRNAs. Several alternatively spliced transcript variants encoding multiple isoforms have been found for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for EEF1D Antibody (H00001936-B01P)(1)

Publication using H00001936-B01P

• Veremieva M, Khoruzhenko A, Zaicev S et al. Unbalanced expression of the translation complex eEF1 subunits in human cardioesophageal carcinoma. Eur J Clin Invest 2010-10-21 [PMID: 20964681]

Bioinformatics

• Gene Symbol: EEF1D
• Entrez:
  • 1936(Human)
• Uniprot:
  • NP_001951.2(Human)