EDAR Antibody (EDAR12) [mFluor Violet 610 SE]

• Reactivity: Hu, Mu, Rt, Ca, Ch
• Applications: WB, ELISA, ICC/IF, B/N, SPR
• Clone: EDAR12
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: mFluor Violet 610 SE

EDAR Antibody (EDAR12) [mFluor Violet 610 SE] Summary

• Description: This conjugate is made on demand and is not held in inventory. Each order represents a unique lot. All conjugates are generated using validated conjugation protocols and meet strict degree of labeling (F/P) specifications; additional information is available upon request. The antibody concentration is provided on the individual product label.
  
  For specialized conjugation needs, like large quantities, specific concentrations, and defined F/P ratios, bulk and custom antibody conjugation services are available.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: EDAR12 was prepared by immunixzing female OVE1B mice (with the Edar gene deleted) subcutaneously with mouse EDAR-Fc and positive hybridoma clones were screened for binding mEDAR by ELISA.
• Specificity: EDAR12 recognises and binds to CRD1 of the extracellular domain of mouse EDAR. EDAR3 cross-reacts with EDAR derived from human, dog, rat and chicken when EDAR is fused to the glycosylphosphatidylinositol anchor of TRAILR3. EDAR is the receptor for the TNF family ligand EDA1, which is a type II transmembrane protein possessing a collagen-like domain and a C-terminal TNF-homology domain. EDAR is important for the correct development of skin appendages including hair, teeth and eccrine sweat glands. LoF mutations in the Eda gene is known to cause XLHED (X-linked hypohidrotic ectodermal dysplasia), and results in abnormal development.
• Isotype: IgG Kappa
• Clonality: Monoclonal
• Host: Rabbit
• Gene: EDAR
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Block/Neutralize
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Surface Plasmon Resonance
  • Western Blot
• Application Notes: Recommended applications are based on validated applications from the unconjugated base product NBP2-81103. This conjugated antibody is not kept in inventory and is made to order using validated conjugation protocols.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Protein A purified

Notes

mFluor(TM) is a trademark of AAT Bioquest, Inc. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for EDAR Antibody (EDAR12) [mFluor Violet 610 SE]

• Anhidrotic ectodysplasin receptor 1
• DLED3
• Ectodermal dysplasia receptor
• ectodysplasin 1, anhidrotic receptor
• ectodysplasin A receptor
• Ectodysplasin-A receptor
• ED1R
• ED5
• EDA1R
• EDA3EDA-A1 receptor
• EDA-A1R
• EDAR
• FLJ94390
• HRM1
• mouse, homolog of
• tumor necrosis factor receptor superfamily member EDAR

Background

The EDAR gene encodes a 448 amino acid long, approximately 48 kDA tumor necrosis factor receptor superfamily member EDAR protein that monitors the activation of NF-kappa-B and JNK. It functions as a receptor for EDA isoform 1 but not for isoform 2. EDAR is critical for hair, teeth and other ectodermal derivatives development. EDAR participates in the TNF superfamily pathway, the integrated breast cancer pathway, and the cytokine-cytokine receptor interaction. It is known to interact with genes EDA, TRAF1, TRAF2, TRAF3, as well as EDARADD. Defects in EDAR cause ectodermal dysplasia 10A and 10B. EDAR is linked to hepatitis, immunodeficiency, osteosarcoma, hypotrichosis, tooth agenesis, breast cancer, Clouston syndrome, and ectodermal dysplasia.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: EDAR