ECAT1 Overexpression Lysate (Native)

Images

 
Western Blot: C6orf221 Overexpression Lysate (Adult Normal) [NBL1-08528] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for C6orf221.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

ECAT1 Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC215631 and based on accession number NM_001017361. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens chromosome 6 open reading frame 221 (C6orf221), mRNA.
Gene
KHDC3L

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
24.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for ECAT1

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for ECAT1 Overexpression Lysate (Native)

  • C6orf221
  • chromosome 6 open reading frame 221
  • ECAT1 ES cell associated transcript 1
  • ES cell-associated transcript 1 protein
  • HYDM2
  • KH domain containing 3-like, subcortical maternal complex member

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP2-30762
Species: Hu
Applications: IHC, IHC-P
NBP1-88798
Species: Hu
Applications: ICC/IF, IHC, IHC-P
NBP1-76290
Species: Hu
Applications: WB, ELISA, ICC/IF, IHC, IF
H00388112-B01P
Species: Hu
Applications: WB, ELISA
AF1997
Species: Hu
Applications: WB, ChIP, ICC
MAB4645
Species: Hu
Applications: CyTOF-ready, ICC, ICFlow
NBP2-30762
Species: Hu
Applications: IHC, IHC-P
AF3125
Species: Hu
Applications: WB, ICC
NB100-56745
Species: Hu, Mu
Applications: WB, ChIP, ICC/IF, IP
NBP1-76939
Species: Hu, Mu, Rt
Applications: WB, ELISA, ICC/IF
AF5760
Species: Hu
Applications: WB, IHC
H00079816-M01
Species: Hu
Applications: WB, ELISA, ICC/IF, IHC
NB100-56155
Species: Hu
Applications: WB, IHC, IHC-P, IP
NB100-1831
Species: Hu, Mu, Rt
Applications: WB, Simple Western, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
MAB2018
Species: Hu, Mu, Rt
Applications: WB, Simple Western, CyTOF-reported, ICC, ICFlow
NBL1-08528
Species: Hu
Applications: WB

Publications for ECAT1 Lysate (NBL1-08528) (0)

There are no publications for ECAT1 Lysate (NBL1-08528).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for ECAT1 Lysate (NBL1-08528) (0)

There are no reviews for ECAT1 Lysate (NBL1-08528). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for ECAT1 Lysate (NBL1-08528) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional ECAT1 Products

Bioinformatics Tool for ECAT1 Lysate (NBL1-08528)

Discover related pathways, diseases and genes to ECAT1 Lysate (NBL1-08528). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Blogs on ECAT1

There are no specific blogs for ECAT1, but you can read our latest blog posts.

Customers Who Bought This Also Bought

ECAT1 Antibody
NBP1-91737

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our ECAT1 Overexpression Lysate (Native) and receive a gift card or discount.

Bioinformatics

Gene Symbol KHDC3L