EBI2/GPR183 Antibody - BSA Free

Product Discontinued

EBI2/GPR183 Antibody - BSA Free Summary

• Description: Product can be stored undiluted at 4C for up to 1 month.
• Immunogen: Synthetic 20 amino acid peptide from 3rd cytoplasmic domain of human EBI2/GPR183.
• Epitope: 3rd cytoplasmic domain
• Localization: Multi-pass membrane protein
• Specificity: Human EBI2/GPR183. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
• Predicted Species: Porcine (95%), Bovine (95%), Rabbit (95%), Canine (95%), Primate (100%), Equine (95%), Monkey (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: GPR183
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 5 - 10 ug/ml
• Control:

  EBI2/GPR183 Overexpression Lysate

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Gorilla (100%), Marmoset (95%), Hamster (85%), Bat (85%), Chicken (85%).

Packaging, Storage & Formulations

• Storage: Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.1% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for EBI2/GPR183 Antibody - BSA Free

• EBI2
• EBI2Epstein-Barr virus induced gene 2 (lymphocyte-specific G protein-coupledreceptor)
• EBV-induced G-protein coupled receptor 2
• Epstein-Barr virus-induced G-protein coupled receptor 2
• G protein-coupled receptor 183
• GPR183
• G-protein coupled receptor 183

Background

EBI2 is an Orphan-A GPCR with an unknown ligand. It has a probable role as a mediator of EBV effects on B-lymphocyte and lymphoid tissues but not in T-lymphocyte or peripheral-blood T lymphocytes. EBI2 expression has been documented in B-lymphocyte cell lines and in lymphoid and pulmonary tissues. ESTs have been isolated from normal blood, breast, vessel, thymus, tonsil, lymph, ear, and fetal lung, testis, and B-cell libraries. ESTs have also been isolated from blood, bone marrow, breast, ear, ganglion, lung, lymph node, placenta, thymus, tonsil, and vessel cancer libraries.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

FAQs for EBI2/GPR183 Antibody (NLS46). (Showing 1 - 1 of 1 FAQ).

1. Has the anti-EBI2 antibody cat.# NLS46 been tested for flow cytometry? We have generated transfectants expressing human EBI2 (and a GFP reporter) and would be keen on testing it if this has not been done. Would you be able to provide us with a small sample?
   • We have not tested this antibody in FLOW application yet, but I do not see any reason why it should not work in FLOW. In fact, as the immunogen for this antibody corresponds to the extracellular domain of the target protein and the antibody performed well in IHC-P, I strongly believe that the antibody must work in FLOW application also. We cannot guarantee its FLOW application, however, you may optimize the best suitable conditions for the assay from your own testing, and we would be more than happy to provide you our troubleshooting/technical feedback if you come up with any problems in generating your desired outcome. If you would be interested in testing this novel application, please take a look at our Innovator's Reward program.

Bioinformatics

• Gene Symbol: GPR183
• Entrez:
  • 1880(Human)