EBI2/GPR183 Antibody (486617) [Unconjugated]

HEK293 human embryonic kidney cell line transfected with either (A) human EBI2/GPR183 or (B) irrelevant protein, and eGFP was stained with Mouse Anti-Human EBI2/GPR183 Monoclonal Antibody (Catalog # MAB102721) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based on isotype control antibody (Catalog # MAB0041, data not shown). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Hu
• Applications: Flow, CyTOF-ready
• Clone: 486617
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

EBI2/GPR183 Antibody (486617) [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived transfected with recombinant human EBI2
  Accession # P32249
• Specificity: Detects human EBI2 in direct ELISAs.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for EBI2/GPR183 Antibody (486617) [Unconjugated]

• EBI2
• EBI2Epstein-Barr virus induced gene 2 (lymphocyte-specific G protein-coupledreceptor)
• EBV-induced G-protein coupled receptor 2
• Epstein-Barr virus-induced G-protein coupled receptor 2
• G protein-coupled receptor 183
• GPR183
• G-protein coupled receptor 183

Background

Epstein-Barr virus-induced G-protein coupled receptor 2, also known as EBI2 or GPR183 is a G-protein coupled receptor (GPCR) expressed in lymphocytes that acts as a chemotactic receptor for B-cells, T-cells, splenic dendritic cells, and monocytes/macrophages. The GPR183 gene was identified by the up-regulation of its expression upon Epstein-Barr virus infection of the Burkitt's lymphoma cell line BL41. GPR183 collaborates with CXCR5 to mediate B cell homing within a lymph node, probably by forming a heterodimer with CXCR5 that affects its interaction with CXCL13

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • P32249()