DUSP8 Antibody

Product Discontinued

DUSP8 Antibody Summary

• Immunogen: Peptide with sequence AGDRLPRKVMDAK-C corresponding to N-Terminus according to NP_004411.2.
• Specificity: This antibody is also expected to recognise the hypothetical human protein similar to dual specificity phosphatase 8 (XM_114902), which is virtually identical.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: DUSP8
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:4000
  • Western Blot 1 - 3 ug/ml
• Application Notes: WB: Approx. 60-65 kDa band bands observed in human heart lysate.

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Mouse, Rat, Bovine.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris (pH 7.3), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for DUSP8 Antibody

• C11orf81
• chromosome 11 open reading frame 81
• dual specificity phosphatase 8
• Dual specificity protein phosphatase hVH-5
• EC 3.1.3.16
• EC 3.1.3.48
• FLJ42476
• FLJ42958
• HVH8
• serine/threonine specific protein phosphatase
• vaccinia virus homolog
• VH5

Background

DUSP8 is a member of the dual specificity protein phosphatase subfamily. These phosphatases inactivate their target kinases by dephosphorylating both the phosphoserine/threonine and phosphotyrosine residues. They negatively regulate members of the mitogen-activated protein (MAP) kinase superfamily (MAPK/ERK, SAPK/JNK, p38), which is associated with cellular proliferation and differentiation. Different members of the family of dual specificity phosphatases show distinct substrate specificities for various MAP kinases, different tissue distribution and subcellular localization, and different modes of inducibility of their expression by extracellular stimuli. DUSP8 inactivates SAPK/JNK and p38, is expressed predominantly in the adult brain, heart, and skeletal muscle, is localized in the cytoplasm, and is induced by nerve growth factor and insulin. An intronless pseudogene for DUSP8 is present on chromosome 10q11.2.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for DUSP8 Antibody (NB100-886)(1)

Publication using NB100-886

• Martell KJ, Seasholtz AF, Kwak SP et al. hVH-5: a protein tyrosine phosphatase abundant in brain that inactivates mitogen-activated protein kinase. J Neurochem 1995-10-01 [PMID: 7561881]

Bioinformatics

• Gene Symbol: DUSP8
• Entrez:
  • 1850(Human)
  • 18218(Mouse)
• Uniprot:
  • NP_004411(Human)