Recombinant Human DUSP18 His Protein

SDS-Page: Recombinant Human DUSP18 Protein [NBP1-99084] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain

• Reactivity: Hu
• Applications: PAGE
• Concentration: 0.5 mg/ml

Recombinant Human DUSP18 His Protein Summary

• Description: A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-188 of Human DUSP18
  
  

  Source: E.coli

  Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSHMTAPSC AFPVQFRQPS VSGLSQITKS LYISNGVAAN NKLMLSSNQI TMVINVSVEV VNTLYEDIQY MQVPVADSPN SRLCDFFDPI ADHIHSVEMK QGRTLLHCAA GVSRSAALCL AYLMKYHAMS LLDAHTWTKS CRPIIRPNSG FWEQLIHYEF QLFGKNTVHM VSSPVGMIPD IYEKEVRLMI PL

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: DUSP18
• Purity: >95%, by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • SDS-Page
• Theoretical MW: 23.6 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20 mM Tris-HCl buffer (pH 8.0), 1 mM DTT, 40% glycerol, 0.1 mM PMSF, 1 mM EDTA
• Preservative: No Preservative
• Concentration: 0.5 mg/ml
• Purity: >95%, by SDS-PAGE

Alternate Names for Recombinant Human DUSP18 His Protein

• bK963H5.1
• DSP18
• dual specificity phosphatase 18
• dual specificity protein phosphatase 18
• DUSP20
• EC 3.1.3.16
• EC 3.1.3.48
• LMWDSP20
• LMW-DSP20
• Low molecular weight dual specificity phosphatase 20
• MGC32658

Background

Dual specificity phosphatase 18, also known as DUSP18, is a member of the dual-specificity phosphatase (DSP) family, which catalyzes dephosphorylation of phosphotyrosine and phosphothreonine residues. DUSP18 is inhibited by iodoarectic acid and is activated by manganese ions. Along with having preferential enzymatic activity against phosphorylated tyrosine residues over threonine residues, DUSP18 also dephosphorylates p-nitrophenyl phosphate (pNPP) in vitro. This protein is widely expressed with highest levels in liver, brain, ovary and testis. Recombinant human DUSP18 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: DUSP18