DUSP18 Overexpression Lysate (Native)

Images

 
Western Blot: DUSP18 Overexpression Lysate (Adult Normal) [NBP2-05357] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for DUSP18.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

DUSP18 Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC215316 and based on accession number NP_689724. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens DUSP18, mRNA.
Gene
DUSP18

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
20.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for DUSP18

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for DUSP18 Overexpression Lysate (Native)

  • bK963H5.1
  • DSP18
  • dual specificity phosphatase 18
  • dual specificity protein phosphatase 18
  • DUSP20
  • EC 3.1.3.16
  • EC 3.1.3.48
  • LMWDSP20
  • LMW-DSP20
  • Low molecular weight dual specificity phosphatase 20
  • MGC32658

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP1-49879
Species: Hu
Applications: WB, IHC, IHC-P, PEP-ELISA
46780002
Species: Hu
Applications: ELISA
MAB1654
Species: Hu, Mu, Rt
Applications: WB
NBP1-68874
Species: Hu, Mu, Rt
Applications: WB, PEP-ELISA
NB500-138
Species: Hu
Applications: WB, IHC, IHC-P
NBP1-81575
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-88419
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
MAB17761
Species: Hu, Mu, Rt
Applications: WB, ICC
NBP2-37568
Species: Hu
Applications: WB, ELISA, Flow, ICC/IF, IHC
NBP2-00764
Species: Hu
Applications: WB, Flow, IHC, IHC-P
MAB4457
Species: Hu, Mu, Rt
Applications: WB
NBP2-01245
Species: Hu
Applications: WB, Flow, ICC/IF, IHC, IHC-P
NBP1-86496
Species: Hu
Applications: ICC/IF, IHC, IHC-P
H00063904-B01P
Species: Hu
Applications: WB, ELISA
NBP2-42173
Species: Hu
Applications: WB, ICC/IF, ICC
NB100-834
Species: Hu
Applications: WB, IHC, IHC-P, PEP-ELISA
NBP2-30075
Species: Hu
Applications: WB, Flow, ICC/IF, IHC-P
NBP2-05357
Species: Hu
Applications: WB

Publications for DUSP18 Lysate (NBP2-05357) (0)

There are no publications for DUSP18 Lysate (NBP2-05357).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for DUSP18 Lysate (NBP2-05357) (0)

There are no reviews for DUSP18 Lysate (NBP2-05357). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for DUSP18 Lysate (NBP2-05357) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional DUSP18 Products

Bioinformatics Tool for DUSP18 Lysate (NBP2-05357)

Discover related pathways, diseases and genes to DUSP18 Lysate (NBP2-05357). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Pathways for DUSP18 Lysate (NBP2-05357)

View related products by pathway.

PTMs for DUSP18 Lysate (NBP2-05357)

Learn more about PTMs related to DUSP18 Lysate (NBP2-05357).
 

Research Areas for DUSP18 Lysate (NBP2-05357)

Find related products by research area.

Blogs on DUSP18

There are no specific blogs for DUSP18, but you can read our latest blog posts.

Customers Who Bought This Also Bought

DUSP18 Antibody
NB100-56481

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our DUSP18 Overexpression Lysate (Native) and receive a gift card or discount.

Bioinformatics

Gene Symbol DUSP18