Human DPPIV/CD26 ELISA Kit (Colorimetric)

ELISA: DPPIV/CD26 ELISA Kit [NBP1-91180] - Standard curve

Product Discontinued

Human DPPIV/CD26 ELISA Kit (Colorimetric) Summary

• Description: The human sCD26 ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of human sCD26
• Specificity: The interference of circulating factors of the immune system was evaluated by spiking these proteins at physiologically relevant concentrations into a human DPPIV/CD26 positive serum. No crossreactivity was detected.
• Standard Curve Range: 15.6 - 500 ng/mL
• Sensitivity: 7.3 ng/mL
• Assay Type: Sandwich ELISA
• Inter-Assay: CV% < 9.10%
• Intra-Assay: CV% < 4.60%
• Spike Recovery: 95%
• Sample Volume: 20 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: DPP4

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Human DPPIV/CD26 ELISA Kit (Colorimetric)

• ADABP
• ADCP-2
• ADCP2DPP IV
• Adenosine deaminase complexing protein 2TP103
• CD26 antigen
• CD26
• CD26T-cell activation antigen CD26
• dipeptidyl peptidase 4
• Dipeptidyl peptidase IV
• dipeptidylpeptidase 4
• dipeptidyl-peptidase 4
• dipeptidylpeptidase IV (CD26, adenosine deaminase complexing protein 2)
• DPP4
• DPPIV
• EC 3.4.14.5

Background

D26, a T cell activation antigen, is a 110kDa cell surface glycoprotein with known dipeptidyl peptidase IV activity in its extracellular domain which is present on various cell types, including T cells and epithelial cells of the liver, kidney, and intestine. Of the T cell antigens described to date, CD26 has proved to be one of the most intriguing in the scope of its functional associations. Considerable evidence suggests that CD26 can deliver a potent costimulatory signal to T-cells. This signal transducing property appears to be a property of its extracellular domain. In addition, CD26 appears to be a functional collagen receptor that may aid activated T-cells in localizing to inflammatory regions. It has also been demonstrated that CD26 not only acts as a functional dipeptidyl peptidase IV, but also binds strongly to adenosine deaminase. Significant levels of DPPIV activity have been shown to exist in plasma, serum, and urine. The serum form of DPPIV is unique, and is not a breakdown product of membrane CD26, nevertheless exposing significant structural similarity to the membrane form.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

FAQs for DPPIV/CD26 ELISA Kit (NBP1-91180). (Showing 1 - 1 of 1 FAQ).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: DPP4