DPCR-1 Antibody (986124) [Unconjugated]

DPCR-1 was detected in immersion fixed HT-29 human colon adenocarcinoma cell line (positive control) and not U-87 MG human glioblastoma/astrocytoma cell line (negative control) using Mouse Anti-Human DPCR-1 Monoclonal Antibody (Catalog # MAB33471) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

• Reactivity: Hu
• Applications: ICC/IF
• Clone: 986124
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

DPCR-1 Antibody (986124) [Unconjugated] Summary

• Immunogen: Human DPCR-1 synthetic peptide
  Accession # Q3MIW9
• Specificity: Detects human DPCR-1 in direct ELISAs.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 8-25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for DPCR-1 Antibody (986124) [Unconjugated]

• bCX105N19.6
• C6orf37
• diffuse panbronchiolitis critical region 1
• DPCR1
• DPCR-1
• MGC126710
• MGC126712
• PBLT
• PBLTdiffuse panbronchiolitis critical region 1 protein

Background

DPCR-1 (Diffuse Panbronchiolitis Critical Region 1) is encoded by a gene that maps to the chromosomal diffuse panbronchiolitis critical region. DPCR-1 is associated with susceptibility to this bronchiolar disease. It has homology with the mucin-like domain of zonadhesin. DPCR-1 is predominantly expressed in lung, kidney, and testis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • Q3MIW9()