DORFIN Antibody - BSA Free

Immunoprecipitation: DORFIN Antibody [NBP1-71860] - Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Affinity purified rabbit anti-Dorfin antibody used for IP at 6 mcg/mg lysate. Dorfin was also immunoprecipitated by another rabbit anti-Dorfin antibody which recognizes an upstream epitope.

Product Discontinued

DORFIN Antibody - BSA Free Summary

• Immunogen: The immunogen for this product maps to a region between residue 600 and 650 of human Double Ring-Finger Protein using the numbering given in entry NP_056250.3 (GeneID 25897).
• Predicted Species: Porcine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: RNF19A
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 2 - 10 ug/mg lysate
• Application Notes: Western Blot Not recommended.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for DORFIN Antibody - BSA Free

• DKFZp566B1346
• dorfin
• Double ring-finger protein
• E3 ubiquitin-protein ligase RNF19A
• EC 6.3.2
• EC 6.3.2.-
• p38
• protein p38 interacting with transcription factor Sp1
• RING finger protein 19 isoform
• ring finger protein 19
• ring finger protein 19Ap38 protein
• ring-IBR-ring domain containing protein Dorfin
• RNF19

Background

RNF19A contains two RING-finger motifs and an IBR (in between RING fingers) motif. This protein is an E3 ubiquintin ligase that is localized in Lewy bodies (LBs), a characteristic neuronal inclusion in Parkinson's disease (PD) brains. This protein interacts with UBE2L3/UBCH7 and UBE2E2/UBCH8, but not other ubiquitin-conjugating enzymes. This protein is found to bind and ubiquitylate synphilin 1 (SNCAIP), which is a interacting protein of alpha synuclein in neurons, and a major component of LB.The protein encoded this gene contains two RING-finger motifs and an IBR (in between RING fingers) motif. This protein is an E3 ubiquintin ligase that is localized in Lewy bodies (LBs), a characteristic neuronal inclusion in Parkinson's disease (PD) brains. This protein interacts with UBE2L3/UBCH7 and UBE2E2/UBCH8, but not other ubiquitin-conjugating enzymes. This protein is found to bind and ubiquitylate synphilin 1 (SNCAIP), which is a interacting protein of alpha synuclein in neurons, and a major component of LB. Alternatively spliced transcript variants encoding the same protein have been reported.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: RNF19A
• Uniprot:
  • NP_056250.3(Human)