Home » DOG1/TMEM16A » DOG1/TMEM16A Antibodies » DOG1/TMEM16A Antibody (DG1/447)

DOG1/TMEM16A Antibody (DG1/447)

 Submit a Review

Images

 
(
Enlarge)
Immunohistochemistry-Paraffin: DOG1/TMEM16A Antibody (DG1/447) [NBP2-44378] - Human GIST stained with DOG1 Monoclonal Antibody (DG1/447).
Immunohistochemistry-Paraffin: DOG1/TMEM16A Antibody (DG1/447) [NBP2-44378] - Human GIST stained with DOG1 Monoclonal Antibody (DG1/447).

Product Details

Summary
Reactivity HuSpecies Glossary
Applications IHC, IHC-P
Clone
DG1/447
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.2 mg/ml

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-34603AF350
Alexa Fluor 405
NBP2-34603AF405
Alexa Fluor 488
NBP2-34603AF488
Alexa Fluor 532
NBP2-34603AF532
Alexa Fluor 594
NBP2-34603AF594
Alexa Fluor 647
NBP2-34603AF647
Alexa Fluor 700
NBP2-34603AF700
Alexa Fluor 750
NBP2-34603AF750
Biotin
NBP2-34603B
DyLight 350
NBP2-34603UV
DyLight 405
NBP2-34603V
DyLight 488
NBP2-34603G
DyLight 550
NBP2-34603R
DyLight 594
NBP2-34603DL594
DyLight 650
NBP2-34603C
DyLight 680
NBP2-34603FR
DyLight 755
NBP2-34603IR
FITC
NBP2-34603F
HRP
NBP2-34603H
Janelia Fluor 549
NBP2-34603JF549
Janelia Fluor 646
NBP2-34603JF646
View Available Formulations
Catalog# & Formulation Size Price
IHC-Prediluted
NBP2-48152
Azide and BSA Free
NBP2-34603-0.1mg
100% Guarantee on every product

DOG1/TMEM16A Antibody (DG1/447) Summary

Immunogen
Recombinant human canine1/TMEM16A protein (Uniprot: Q5XX6)
Localization
Cell Surface and Cytoplasmic
Marker
Gastrointestinal Stromal Tumor Marker
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
ANO1
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Theoretical MW
114 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C.
Buffer
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Purity
Protein A or G purified

Notes

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-34603)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. Antibody is stable for 24 months. Non-hazardous.

Alternate Names for DOG1/TMEM16A Antibody (DG1/447)

  • ANO1
  • anoctamin 1
  • DOG1
  • ORAOV2
  • TAOS2
  • TMEM16A

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

CD117/c-kit
Antibody [Unconjugate
AF1356
Simple Western lane view shows lysates of MO7e human megakaryocytic leukemic cell line, P815 mouse mastocytoma cell line and MC/9-2 mouse mast cell line, loaded at 0.2 mg/mL. A specific band was detected for CD117/c-kit at approximately 150-165 kDa (as indicated) using 5 µg/mL of Goat Anti-Human/Mouse CD117/c-kit Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1356) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.        
Western blot shows lysates of MO7e human megakaryocytic leukemic cell line, P815 mouse mastocytoma cell line, and MC/9‑2 mouse mast cell line. PVDF membrane was probed with 0.1 µg/mL of Goat Anti-Human/Mouse CD117/c-kit Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1356) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). Specific bands were detected for CD117/c-kit at approximately 135, 150 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
CD117/c-kit was detected in immersion fixed frozen sections of mouse embryo using Goat Anti-Human/Mouse CD117/c-kit Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1356) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
(4 Reviews)
(51 Publications)
Species: Hu, Mu
Applications: CyTOF-ready, Flow, IHC, Simple Western, WB
CLCA1 Antibody
NBP2-38869
Immunohistochemistry: CLCA1 Antibody [NBP2-38869] - Staining of human duodenum shows strong cytoplasmic positivity in glandular cells / Goblet cells strongly stained.
Immunohistochemistry-Paraffin: CLCA1 Antibody [NBP2-38869] - Staining of human liver shows low expression as expected.
Immunohistochemistry-Paraffin: CLCA1 Antibody [NBP2-38869] - Staining of human small intestine shows high expression.
Species: Hu
Applications: IHC, IHC-P
CLCA4 Antibody
NBP2-92365
Western Blot: CLCA4 Antibody [NBP2-92365] - Analysis of extracts of various cell lines, using CLCA4 at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 30s.
Western Blot: CLCA4 Antibody [NBP2-92365] - Analysis of extracts of Rat uterus, using CLCA4 at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.
Species: Hu, Rt
Applications: WB
CFTR Antibody
(24-1) [Unconjugated] ...
MAB25031
CFTR was detected in immersion fixed paraffin-embedded sections of human placenta using 8 µg/mL Mouse Anti-Human CFTR C‑Terminus Monoclonal Antibody (Catalog # MAB25031) overnight at 4 °C. Tissue was stained with the Anti-Mouse HRP-AEC Cell & Tissue Staining Kit (red; Catalog # CTS003) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
CFTR was detected in immersion fixed paraffin-embedded sections of human placenta using Mouse Anti-Human CFTR C-Terminus Monoclonal Antibody (Catalog # MAB25031) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
(10 Reviews)
(45 Publications)
Species: Hu
Applications: IHC, IP, WB
PDGFR alpha
Antibody [Unconjugate
AF1062
Western blot shows lysates of mouse uterus tissue and mouse lung tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse PDGF R alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1062) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). Specific bands were detected for PDGF R alpha at approximately 160-200 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
PDGF R alpha was detected in immersion fixed frozen sections of mouse embryo using 15 µg/mL Goat Anti-Mouse PDGF R alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1062) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to the plasma membrane of mesenchymal cells. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Recombinant Human PDGF‑AA (Catalog # 221-AA) stimulates proliferation in the NR6R‑3T3 mouse fibroblast cell line in a dose-dependent manner (orange line), as measured by Resazurin (Catalog # AR002. Proliferation elicited by Recombinant Human PDGF‑AA (250 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse PDGF R alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1062). The ND50 is typically 0.2-1.6 µg/mL.
(4 Reviews)
(115 Publications)
Species: Mu
Applications: IHC, Neut, WB
CD34 Antibody (MEC
14.7)
NB600-1071
Flow Cytometry: CD34 Antibody (MEC 14.7) [NB600-1071] - An intracellular stain was performed on Raw264.7 cells with CD34 Antibody [MEC 14.7] NB600-1071 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by Rat IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 488 (SA5-10018, Thermo Fisher).
Flow Cytometry: CD34 Antibody (MEC 14.7) [NB600-1071] - CD34 (MEC 14.7) antibody was tested at 1:250 in WEHI-3 cells with DyLight 488 (green) alongside a matched isotype control (black).
Immunocytochemistry/Immunofluorescence: CD34 Antibody (MEC 14.7) [NB600-1071] - CD34 antibody was tested in WEHI-3 cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).
(24 Publications)
Species: Hu(-), Mu
Applications: ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, WB
ANO6 Antibody
NBP2-92052
Western Blot: ANO6 Antibody [NBP2-92052] - Analysis of extracts of various cell lines, using ANO6 at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.
Species: Hu, Mu, Rt
Applications: WB
Bestrophin 1
Antibody (E6-6) - BSA
NB300-164
Immunohistochemistry-Paraffin: Bestrophin 1 Antibody (E6-6) [NB300-164] - Bestrophin 1 was detected in immersion fixed paraffin-embedded sections of human brain using Mouse Anti-Human Bestrophin 1 (E6-6) Monoclonal Antibody (Catalog # NB300-164) at 1:300 for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm in neurons.
Immunohistochemistry-Paraffin: Bestrophin 1 Antibody (E6-6) [NB300-164] - Bestrophin 1 was detected in immersion fixed paraffin sections of human small intestine using t Mouse Anti-Human Bestrophin 1 Monoclonal Antibody (Catalog # NB300-164) at 5 ug/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the cell surface and extracellular.
Dual RNAscope ISH-IHC: Bestrophin 1 Antibody (E6-6) [NB300-164] - Formalin-fixed paraffin-embedded tissue sections of human duodenum were probed for Bestrophin 1 mRNA (ACD RNAScope Probe, catalog # 433181; Fast Red chromogen, ACD catalog # 322360). Adjacent tissue section was processed for immunohistochemistry using mouse anti-human (Novus Biologicals catalog # NB300-164) at 0.3ug/mL with overnight incubation at 4 degrees Celsius followed by incubation with anti-mouse IgG VisUCyte HRP Polymer Antibody (Catalog # VC001) and DAB chromogen (yellow-brown). Tissue was counterstained with hematoxylin (blue). IHC signal is confined to cytoplasm.
(1 Review)
(45 Publications)
Species: Ca, Hu, Mu, Po, Pm, Rt(-)
Applications: Dual ISH-IHC, ICC/IF, IHC, IHC-Fr, IHC-P, IP, PLA, WB
G protein alpha
inhibitor 1 Antibody ...
H00002770-M01
Western Blot: G protein alpha inhibitor 1 Antibody (2B8-2A5) [H00002770-M01] - Analysis of GNAI1 expression in transfected 293T cell line by GNAI1 monoclonal antibody (M01), clone 2B8-2A5.Lane 1: GNAI1 transfected lysate(40.4 KDa).Lane 2: Non-transfected lysate.
Immunohistochemistry-Paraffin: G protein alpha inhibitor 1 Antibody (2B8-2A5) [H00002770-M01] - Analysis of monoclonal antibody to GNAI1 on formalin-fixed paraffin-embedded human tonsil. Antibody concentration 3 ug/ml.
Sandwich ELISA: G protein alpha inhibitor 1 Antibody (2B8-2A5) [H00002770-M01] - Detection limit for recombinant GST tagged GNAI1 is approximately 1ng/ml as a capture antibody.
(1 Publication)
Species: Hu
Applications: ELISA, IHC, IHC-P, S-ELISA, WB
IL-4 [Unconjugated]
6507-IL/CF
Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. The ED50 for this effect is 0.05-0.2 ng/mL.
(3 Publications)
Species: Hu
Applications: BA
TMEM16K Antibody
NBP1-59667
Western Blot: TMEM16K Antibody [NBP1-59667] - 293T cells lysate, concentration 0.2-1 ug/ml.
Western Blot: TMEM16K Antibody [NBP1-59667] - Human Fetal Heart, Antibody Dilution: 1.0 ug/ml.
Western Blot: TMEM16K Antibody [NBP1-59667] - Human Adult Placenta, Antibody Dilution: 1.0 ug/ml.
Species: Hu
Applications: WB
ANO2 Antibody -
BSA Free
NBP2-81703
Western Blot: ANO2 Antibody [NBP2-81703] - Western blot analysis of ANO2 in rat brain tissue lysate with ANO2 antibody at (A) 1 and (B) 2 ug/mL
Immunohistochemistry: ANO2 Antibody [NBP2-81703] - Immunohistochemistry of ANO2 in human brain tissue with ANO2 antibody at 5 ug/mL.
Immunocytochemistry/Immunofluorescence: ANO2 Antibody [NBP2-81703] - Immunofluorescence of ANO2 in Human Brain cells with ANO2 antibody at 20 ug/mL.
Species: Hu, Mu, Rt
Applications: ELISA, ICC/IF, IHC, IHC-P, WB
Desmin Antibody
AF3844
Desmin was detected in immersion fixed frozen sections of mouse skeletal muscle using Human Desmin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3844) at 10 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to z-lines. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.
Desmin was detected in immersion fixed paraffin-embedded sections of human heart using 15 µg/mL Human Desmin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3844) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to the sarcoplasm of cardiomyocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
(2 Reviews)
(16 Publications)
Species: Hu, Mu
Applications: IHC
ERMAP Antibody
AF4928
K562 human chronic myelogenous leukemia cell line was stained with Goat Anti-Human ERMAP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4928, filled histogram) or control antibody (Catalog # AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108).
Species: Hu
Applications: CyTOF-ready, Flow, WB

Publications for DOG1/TMEM16A Antibody (NBP2-44378) (0)

There are no publications for DOG1/TMEM16A Antibody (NBP2-44378).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for DOG1/TMEM16A Antibody (NBP2-44378) (0)

There are no reviews for DOG1/TMEM16A Antibody (NBP2-44378). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for DOG1/TMEM16A Antibody (NBP2-44378). (Showing 1 - 1 of 1 FAQ).

  1. Would you please confirm whether NBP2-44378 can be used in intracellular or extracellular flow cytometry?
    • According to human protein atlas, DOG1/TMEM16A/ANO1 has cytoplasmic and membranous expression in several tissues.https://www.proteinatlas.org/ENSG00000131620-ANO1/tissueWe would recommend following our intracellular staining protocol for cytoplasmic targets, which can be found here: https://www.novusbio.com/support/support-by-application/flow-cytometry/protocol.html

Secondary Antibodies

 

Isotype Controls

Additional DOG1/TMEM16A Products

Bioinformatics Tool for DOG1/TMEM16A Antibody (NBP2-44378)

Discover related pathways, diseases and genes to DOG1/TMEM16A Antibody (NBP2-44378). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for DOG1/TMEM16A Antibody (NBP2-44378)

Discover more about diseases related to DOG1/TMEM16A Antibody (NBP2-44378).
 

Pathways for DOG1/TMEM16A Antibody (NBP2-44378)

View related products by pathway.

PTMs for DOG1/TMEM16A Antibody (NBP2-44378)

Learn more about PTMs related to DOG1/TMEM16A Antibody (NBP2-44378).

Blogs on DOG1/TMEM16A

There are no specific blogs for DOG1/TMEM16A, but you can read our latest blog posts.
Omicron Variant Antibodies

Customers Who Bought This Also Bought

Contact Information

Download our Western Blot eHandbook

Product PDFs

  SDS
  PDF Datasheet
  Request CofA

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our DOG1/TMEM16A Antibody (DG1/447) and receive a gift card or discount.


Bioinformatics

Gene Symbol ANO1