DOG1/TMEM16A Antibody (DG1/447)

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Immunohistochemistry-Paraffin: DOG1/TMEM16A Antibody (DG1/447) [NBP2-44378] - Human GIST stained with DOG1 Monoclonal Antibody (DG1/447).

Product Details

Summary
Reactivity HuSpecies Glossary
Applications IHC, IHC-P
Clone
DG1/447
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.2 mg/ml

Order Details

DOG1/TMEM16A Antibody (DG1/447) Summary

Immunogen
Recombinant human canine1/TMEM16A protein (Uniprot: Q5XX6)
Localization
Cell Surface and Cytoplasmic
Marker
Gastrointestinal Stromal Tumor Marker
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
ANO1
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Theoretical MW
114 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C.
Buffer
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Purity
Protein A or G purified

Notes

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-34603)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. Antibody is stable for 24 months. Non-hazardous.

Alternate Names for DOG1/TMEM16A Antibody (DG1/447)

  • ANO1
  • anoctamin 1
  • DOG1
  • ORAOV2
  • TAOS2
  • TMEM16A

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for DOG1/TMEM16A Antibody (NBP2-44378) (0)

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Product General Protocols

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FAQs for DOG1/TMEM16A Antibody (NBP2-44378). (Showing 1 - 1 of 1 FAQ).

  1. Would you please confirm whether NBP2-44378 can be used in intracellular or extracellular flow cytometry?
    • According to human protein atlas, DOG1/TMEM16A/ANO1 has cytoplasmic and membranous expression in several tissues.https://www.proteinatlas.org/ENSG00000131620-ANO1/tissueWe would recommend following our intracellular staining protocol for cytoplasmic targets, which can be found here: https://www.novusbio.com/support/support-by-application/flow-cytometry/protocol.html

Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for DOG1/TMEM16A Antibody (NBP2-44378)

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Bioinformatics

Gene Symbol ANO1