Recombinant Mouse Dihydrofolate Reductase/DHFR His Protein

SDS-Page: Recombinant Mouse Dihydrofolate Reductase/DHFR His Protein [NBP1-72281] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.

Product Discontinued

Recombinant Mouse Dihydrofolate Reductase/DHFR His Protein Summary

• Description: A bioactive recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-187 of Mouse Dihydrofolate Reductase/DHFR
  
  

  Source: E.coli

  Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MVRPLNCIVA VSQNMGIGKN GDLPWPPLRN EFKYFQRMTT TSSVEGKQNL VIMGRKTWFS IPEKNRPLKD RINIVLSREL KEPPRGAHFL AKSLDDALRL IEQPELASKV DMVWIVGGSS VYQEAMNQPG HLRLFVTRIM QEFESDTFFP EIDLGKYKLL PEYPGVLSEV QEEKGIKYKF EVYEKKD

• Details of Functionality: Specific activity is > 2000 pmol/min/ug, and is defined as the amount of enzyme that converts 1.0 pmole of dihydrofolic acid to tetrahydrofolic acid per minute at pH 6.5 at 25C.
• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: DHFR
• Purity: >95%, by SDS-PAGE
• Endotoxin Note: < 1 EU per 1ug of protein (determined by LAL method)

Applications/Dilutions

• Dilutions:
  • Enzyme Activity
  • SDS-Page
• Theoretical MW: 23.8 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20 mM Tris-HCl buffer (pH 8.0), 10% glycerol, 2 mM DTT, 0.1 M NaCl
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: >95%, by SDS-PAGE

Alternate Names for Recombinant Mouse Dihydrofolate Reductase/DHFR His Protein

• DHFR
• DHFRP1
• Dihydrofolate Reductase
• DYR
• EC 1.5.1.3

Background

Dihydrofolate reductase (DHFR) is a member of the reductase family of enzymes that is ubiquitously expressed in all organisms. DHFR catalyzes the NADPH-dependent reduction of dihydrofolate to tetrahydrofolate, and it is essential for the synthesis of thymidylate, purines and several amino acids. Expression of methotrexate (MTX)-resistant variants of DHFR in normal hematopoietic cells is a potential strategy to permit administration of larger doses of MTX by alleviating drug toxicity in normal cells and tissues that are drug sensitive. Recombinant mouse DHFR protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: DHFR