DIAPH3 Antibody

Product Discontinued

DIAPH3 Antibody Summary

• Immunogen: Peptide with sequence C-RTPMPKDVRQS corresponding to internal region (near C-Terminus) according to NP_001035982.1, NP_112194.2.
• Epitope: C-RTPMPKDVRQS
• Specificity: This antibody is expected to recognise both reported isoforms (NP_001035982.1 and NP_112194.2).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: DIAPH3
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:64000
• Application Notes: WB: Preliminary experiments gave an approx. 50 kDa band in human bone marrow, liver and lymph node lysates after 0.3 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 137 kDa band according to NP_001035982.1 and 98.1 kDa band according to NP_112194.2. The ~50 kDa band was successfully blocked by incubation with the immunizing peptide.
• Control:

  DIAPH3 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Notes

Please note, this antibody is considered Innovators Grade. Innovators Grade antibodies are generally unvalidated and require additional characterization for most new species/applications. Novus has made these antibodies available through our Innovators Reward program. Complete an online review with image, detailing your positive or negative results. In return, you receive a discount voucher for 100% of the purchase price of the reviewed product. Please contact us at innovators@novusbio.com for more details.

Alternate Names for DIAPH3 Antibody

• DIAP3
• diaphanous homolog 3 (Drosophila)
• diaphanous-related formin 3
• DKFZp434C0931
• DKFZp686A13178
• homolog) 3
• mDia2
• protein diaphanous homolog 3

Background

DIAPH3 binds to GTP-bound form of Rho and to profilin. Acts in a Rho-dependent manner to recruit profilin to themembrane, where it promotes actin polymerization. It is required for cytokinesis, stress fiber formation, andtranscriptional activation of the serum r

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for DIAPH3 Antibody (NBP1-06031)(1)

Publication using NBP1-06031

• Yang C, Czech L, Gerboth S et al. Novel roles of formin mDia2 in lamellipodia and filopodia formation in motilecells. PLoS Biol 2007-11-01 [PMID: 18044991]

Bioinformatics

• Gene Symbol: DIAPH3
• Entrez:
  • 81624(Human)
• Uniprot:
  • NP_001035982.1(Human)