DHX30 Antibody

Western Blot: DHX30 Antibody [NBP1-26604] - Whole cell lysate from HeLa and 293T cells. DHX30 was also immunoprecipitated by rabbit anti-DHX30 antibody NBP1-26603.

• Reactivity: Hu
• Applications: WB, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

DHX30 Antibody Summary

• Description: Novus Biologicals Rabbit DHX30 Antibody (NBP1-26604) is a polyclonal antibody validated for use in WB and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The immunogen recognized by this antibody maps to a region between residue 1144 and 1194 of human DEAH (Asp-Glu-Ala-His) box polypeptide 30 using the numbering given in entry NP_619520.1 (GeneID 22907).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: DHX30
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 5-10 ug/mg lysate
  • Western Blot 1:2000-1:10000
• Theoretical MW: 134 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: TBS and 0.1% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for DHX30 Antibody

• ATP-dependent RNA helicase DHX30
• DDX30
• DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 30
• DEAH (Asp-Glu-Ala-His) box polypeptide 30
• DEAH box protein 30
• EC 3.6.1
• EC 3.6.4.13
• FLJ11214
• KIAA0890RETCOR
• putative ATP-dependent RNA helicase DHX30
• retina co-repressor

Background

DHX30 (DEAH-box polypeptide 30) is a member of the DEAD box family of proteins that possesses several conserved motifs which include the highly conserved DEAD (Asp-Glu-Ala-Asp) amino acid sequence motif. The major activity of DEAD box proteins is to function as ATP-dependent RNA helicases. As helicases, DEAD proteins play an important role in all aspects of RNA metabolism and function which include pre-mRNA splicing, RNA synthesis, RNA degradation, RNA export, RNA translation, RNA secondary structure formation, ribosome biogenesis, and the assembly of RNP complexes. DHX30 has been implicated to be involved in the packaging of HIV-1 viral RNA and HIV-1 replication.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: DHX30
• Entrez:
  • 22907(Human)
• Uniprot:
  • Q7L2E3(Human)