DHX30 Antibody

Images

 
Independent Antibodies: Western Blot: DHX30 Antibody [NBP1-26603] - Whole cell lysate from HeLa and 293T cells. DHX30 was also immunoprecipitated by rabbit anti-DHX30 antibody NBP1-26604.
Immunoprecipitation: DHX30 Antibody [NBP1-26603] - 5ug antibody used with 500ug B-cell lysate. Same antibody used for western blot 1:2000. 5 minute exposure. Image from verified customer review.

Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, PmSpecies Glossary
Applications WB, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.2 mg/ml

Order Details

DHX30 Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 350 and 400 of human DEAH (Asp-Glu-Ala-His) box polypeptide 30 using the numbering given in entry NP_619520.1 (GeneID 22907).
Predicted Species
Mouse (100%), Rat (100%), Primate (100%), Bovine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
DHX30
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Immunoprecipitation 5-10 ug/mg lysate
  • Western Blot 1:2000-1:10000
Reviewed Applications
Read 3 Reviews rated 2.7
using
NBP1-26603 in the following applications:

Publications
Read Publications using NBP1-26603.

Reactivity Notes

Orangutan (100%).

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Purity
Immunogen affinity purified

Alternate Names for DHX30 Antibody

  • ATP-dependent RNA helicase DHX30
  • DDX30
  • DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 30
  • DEAH (Asp-Glu-Ala-His) box polypeptide 30
  • DEAH box protein 30
  • EC 3.6.1
  • EC 3.6.4.13
  • FLJ11214
  • KIAA0890RETCOR
  • putative ATP-dependent RNA helicase DHX30
  • retina co-repressor

Background

DHX30 (DEAH-box polypeptide 30) is a member of the DEAD box family of proteins that possesses several conserved motifs which include the highly conserved DEAD (Asp-Glu-Ala-Asp) amino acid sequence motif. The major activity of DEAD box proteins is to function as ATP-dependent RNA helicases. As helicases, DEAD proteins play an important role in all aspects of RNA metabolism and function which include pre-mRNA splicing, RNA synthesis, RNA degradation, RNA export, RNA translation, RNA secondary structure formation, ribosome biogenesis, and the assembly of RNP complexes. DHX30 has been implicated to be involved in the packaging of HIV-1 viral RNA and HIV-1 replication.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for DHX30 Antibody (NBP1-26603)(2)

Reviews for DHX30 Antibody (NBP1-26603) (3) 2.73

Average Rating: 2.7
(Based on 3 reviews)
We have 3 reviews tested in 1 species: Human.

Reviews using NBP1-26603:
Filter by Applications
IP
(1)
WB
(1)
ICC
(1)
All Applications
Filter by Species
Human
(3)
All Species
Images Ratings Applications Species Date Details
Immunoprecipitation DHX30 NBP1-26603
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3
reviewed by:
Verified Customer
IP Human 02/19/2019
View

Summary

ApplicationImmunoprecipitation
Sample TestedB-cell lysate
SpeciesHuman
LotA1

Comments

Comments5ug antibody used with 500ug B-cell lysate. Same antibody used for western blot 1:2000. 5 minute exposure
Western Blot DHX30 NBP1-26603
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4
reviewed by:
Verified Customer
WB Human 03/13/2018
View

Summary

ApplicationWestern Blot
Sample TestedLymphocytes
SpeciesHuman
LotA1

Comments

CommentsBIOSCARF
Immunocytochemistry DHX30 NBP1-26603
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1
reviewed by:
Verified Customer
ICC Human 01/11/2018
View

Summary

ApplicationImmunocytochemistry
Sample Testedfibroblasts
SpeciesHuman
LotA1

Comments

Comments1:100 in 1X PBS/1% BSA/0.3% Triton X-100, 16 hours 4C. Staining didn't show localization to mitochondria, looked nuclear/throughout cell. There are other validated antibodies for immunofluorescence. This antibody was purchased for western blot, and I wanted to test it for immunofluorescence.

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for DHX30 Antibody (NBP1-26603). (Showing 1 - 1 of 1 FAQs).

  1. Have you ever tried this antibody on immunohistochemistry on paraffin section?
    • We have not yet tested this product for IHC-P staining. If it had been tested and confirmed it would be listed, and if it had been tested and confirmed not to work, or reported not to work by a customer, we would list the applications as IHC-P (-). If you would like to test this one in the new application and provide feedback on the results I can recommend our Innovators Reward Program.
mFluor Violet Conjugated Antibodies

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Recent Reviews

2.7
5
0
4
1
3
1
2
0
1
1

Verified Customer
02/19/2019
Application: IP
Species: Human

Verified Customer
03/13/2018
Application: WB
Species: Human

Verified Customer
01/11/2018
Application: ICC
Species: Human

Bioinformatics

Gene Symbol DHX30
Entrez
Uniprot