DEFB129 Overexpression Lysate (Native) Summary
The lysate was created in HEK293T cells, using plasmid ID RC210016 and based on accession number NM_080831. The protein contains a C-terminal DDK tag.
Homo sapiens defensin, beta 129 (DEFB129), mRNA.
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Packaging, Storage & Formulations
Store at -80C. Avoid freeze-thaw cycles.
Lysate Details for DEFB129
HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.
Alternate Names for DEFB129 Overexpression Lysate (Native)
- beta defensin 29
- beta-defensin 129
- Beta-defensin 29
- DEFB-29chromosome 20 open reading frame 87
- defensin, beta 129DEFB29
Defensins are cysteine-rich cationic polypeptides that are important in the immunologic response to invading microorganisms. The protein encoded by this gene is secreted and is a member of the beta defensin protein family. Beta defensin genes are found in several clusters throughout the genome, with this gene mapping to a cluster at 20p13.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed
for 6 months from date of receipt.
Customers Who Viewed This Item Also Viewed...
Species: Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, Cell Depl, CyTOF-ready, InhibTFunc
Species: Hu, Po
Applications: Simple Western, ICC/IF, IHC, IHC-P, ICC
Applications: IHC, IHC-P
Applications: WB, ELISA, IHC, IHC-P
Applications: WB, IHC, Neut
Applications: WB, IHC, IP
Species: Hu, Mu, Rt
Applications: WB, ELISA
Applications: WB, ICC/IF, IHC, IHC-P
Applications: Flow, CyTOF-ready
Applications: WB, Simple Western, IHC, CyTOF-ready, ICFlow
Applications: ICC/IF, IHC, IHC-P
Publications for DEFB129 Lysate (NBL1-09825) (0)
There are no publications for DEFB129 Lysate (NBL1-09825).
By submitting your publication information earn gift cards and discounts for future purchases.
Reviews for DEFB129 Lysate (NBL1-09825) (0)
There are no reviews for DEFB129 Lysate (NBL1-09825).
By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
- Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
- Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
- 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen
Product General Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
FAQs for DEFB129 Lysate (NBL1-09825) (0)
Additional DEFB129 Products
Bioinformatics Tool for DEFB129 Lysate (NBL1-09825)
Discover related pathways, diseases and genes to DEFB129 Lysate (NBL1-09825). Need help?
Read the Bioinformatics Tool Guide
for instructions on using this tool.
Blogs on DEFB129