DDX50 Antibody

Images

 
Western Blot: DDX50 Antibody [NB100-60676] - Whole cell lysate (5, 15 and 50 ug) from HeLa cells. Antibody used for WB at 0.04 ug/ml.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.2 mg/ml

Order Details

DDX50 Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 687 and 737 of human DEAD (Asp-Glu-Ala-Asp) box polypeptide 50 using the numbering given in entry NP_076950.1 (GeneID 79009).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
DDX50
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1:2000-1:10000
Positive Control
DDX50 Lysate (NBL1-09802)

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Purity
Immunogen affinity purified

Alternate Names for DDX50 Antibody

  • ATP-dependent RNA helicase DDX50
  • DEAD (Asp-Glu-Ala-Asp) box polypeptide 50
  • DEAD box protein 50
  • EC 3.6.1
  • EC 3.6.4.13
  • GU2
  • GUB
  • gu-beta
  • MGC3199
  • Nucleolar protein Gu2
  • RH-II/GuB
  • RNA helicase II/Gu beta

Background

DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this DEAD box protein family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box enzyme that may be involved in ribosomal RNA synthesis or processing. This gene and DDX21, also called RH-II/GuA, have similar genomic structures and are in tandem orientation on chromosome 10, suggesting that the two genes arose by gene duplication in evolution. This gene has pseudogenes on chromosomes 2, 3 and 4. Alternative splicing of this gene generates multiple transcript variants, but the full length nature of all the other variants but one has not been defined.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol DDX50
Entrez
Uniprot