DCDC2 Antibody

Images

 
Western Blot: DCDC2 Antibody [NB100-2806] - NB100-2806 (0.2ug/ml) staining of COS7 cell lysate transfected with full length recombinant human DCDC2 (A) and untransfected control COS7 cells (B). Primary incubation was 1 ...read more
Immunohistochemistry-Paraffin: DCDC2 Antibody [NB100-2806] - Human Cerebral Cortex shows cytoplasm staining in select neurons.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC, IHC-P, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

DCDC2 Antibody Summary

Immunogen
Peptide with sequence C-DGEKANKDAEQKED corresponding to internal region according to NP_057440.2.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
DCDC2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.1 - 0.3 ug/ml
  • Immunohistochemistry 5 - 10 ug/ml
  • Immunohistochemistry-Paraffin 5 - 10 ug/ml
  • Peptide ELISA Detection limit 1:4000
Application Notes
WB: Full length recombinant human DCDC2 expressed in COS7 (Lane A), non-transfectant COS7 (lane B). Approx. 52 kDa band observed (calculated MW of 52.8 kDa band according to NP_057440.1), provided by Wang and LoTurco, University of Connecticut, USA. An anonymous customer has reported positive results on mouse brain tissue. IHC-P: Human cerebral cortex shows cytoplasm staining in select neurons. Anonymous customers have reported positive results in on rat retinal tissue and rat dorsal root ganglion.
Positive Control
DCDC2 Lysate (NBL1-09739)
Publications
Read Publication using NB100-2806.

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Canine, Porcine.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for DCDC2 Antibody

  • DCDC2A
  • doublecortin domain containing 2
  • KIAA1154Protein RU2S
  • RU2doublecortin domain-containing protein 2
  • RU2S

Background

The DCDC2 protein has two doublecortin peptide domains. This domain has been demonstrated to bind tubulin and enhance microtubule polymerization. Mutations in the gene encoding DCDC2 have been associated with Reading Disability (RD), also referred to as developmental dyslexia.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for DCDC2 Antibody (NB100-2806)(1)

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Product General Protocols

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Video Protocols

WB Video Protocol

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol DCDC2
Entrez
Uniprot