Cystatin B/Stefin B Antibody [Biotin]

• Reactivity: Hu
• Applications: WB
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Biotin
• Concentration: LYOPH

Cystatin B/Stefin B Antibody [Biotin] Summary

• Immunogen: E. coli-derived recombinant human Cystatin B (R&D Systems, Catalog # 1408-PI)
  Met2-Phe98
  Accession # P04080
• Specificity: Detects human Cystatin B in Western blots. In this format, less than 2% cross-reactivity with recombinant mouse Cystatin B, recombinant human (rh) Cystatin A, rhCystatin C, rhCystatin D, rhCystatin E/M, rhCystatin S, rhCystatin SA, and rhCystatin SN is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: CSTB
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 0.1 ug/mL
• Readout System:
  • Streptavidin Native Protein NBP2-29367
  • Streptavidin Native Protein NBP2-29370
  • Streptavidin Native Protein NBP2-29371

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Cystatin B/Stefin B Antibody [Biotin]

• CPI-B
• CST6cystatin B (liver thiol proteinase inhibitor)10STFBcystatin-B
• CSTB
• cystatin B (stefin B)
• Cystatin B
• EPM1
• Liver thiol proteinase inhibitor
• PME
• Stefin B
• stefin-B

Background

Cystatin B, also called stefin B or liver thiol proteinase inhibitor, is a member of family 1 of the cystatin superfamily (1). Like Cystatin A, it is an intracellular inhibitor regulating the activities of cysteine proteases of the papain family such as cathepsins B, H and L (2). Mutations in the Cystatin B gene is the cause of progressive myoclonus epilepsy (EPM1) (3). Because of its expression patterns, Cystatin B can be used as a marker for certain cancers, such as glioblastoma tumors (4). It readily forms amyloid fibrils in vitro (5). The human Cystatin B consists of 98 amino acid residues (3).

1. Abrahamson, M. (1994) Methods Enzymol. 244:685.
2. Pol, E. and I. Bjork (1999) Biochemistry 38:10519.
3. Pennacchio, L.A. et al. (1996) Science 271:1731.
4. Zhang, R. et al. (2003) Glia 42:194.
5. Zerovnik, E. et al. (2002) Biochim. Biophys. Acta 1594:1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CSTB
• Uniprot:
  • P04080()