Cystatin-8 Antibody

Western Blot: Cystatin-8 Antibody [NBP1-49834] - HEK293 lysate (10ug protein in RIPA buffer) overexpressing Human CST8 with C-terminal MYC tag probed with (1ug/ml) in Lane A and probed with anti-MYC Tag (1/1000) in lane C. Mock-transfected HEK293 probed with (1mg/ml) in Lane B. Primary incubations were for 1 hour. Detected by chemiluminescence.

• Reactivity: Hu
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

Cystatin-8 Antibody Summary

• Description: Novus Biologicals Goat Cystatin-8 Antibody (NBP1-49834) is a polyclonal antibody validated for use in WB and ELISA. Anti-Cystatin-8 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence STNEICAIQENSK corresponding to internal region according to NP_005483.1.
• Epitope: STNEICAIQENSK - internal region
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: CST8
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:16000
  • Western Blot 1 - 3 ug/ml
• Application Notes: WB: In transfected HEK293 transiently expressing human CST8 (MYC and DYKDDDDK tagged), a band of approx. 23 kDa is observed. No bands are observed in mock-transfected HEK293 and the same band is observed using anti-myc tag antibody.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Cystatin-8 Antibody

• CRESCystatin-related epididymal spermatogenic protein
• cystatin 8 (cystatin-related epididymal specific)
• cystatin-8
• cystatin-related epididymal-specific

Background

The cystatin superfamily encompasses proteins that contain multiple cystatin-like sequences. Some of the members are active cysteine protease inhibitors, while others have lost or perhaps never acquired this inhibitory activity. There are three inhibitory families in the superfamily, including the type 1 cystatins (stefins), type 2 cystatins and the kininogens. The type 2 cystatin proteins are a class of cysteine proteinase inhibitors found in a variety of human fluids and secretions. The cystatin locus on chromosome 20 contains the majority of the type 2 cystatin genes and pseudogenes. This gene is located in the cystatin locus and encodes a protein similar to type 2 cystatins. The protein exhibits highly tissue-specific expression in the reproductive tract, suggesting implicit roles in reproduction. Alternative splicing identified in mouse is suggested in human based on EST evidence but the full-length nature of putative variants has not been determined.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Cystatin-8 Antibody (NBP1-49834)(1)

Publication using NBP1-49834

• Chapuis J, Hot D, Hansmannel F et al. Transcriptomic and genetic studies identify IL-33 as a candidate gene for Alzheimer's disease. Mol Psychiatry 2009-11-01 [PMID: 19204726]

Bioinformatics

• Gene Symbol: CST8
• Entrez:
  • 10047(Human)
• Uniprot:
  • NP_005483.1(Human)