CYP26A1 Antibody

Immunocytochemistry/Immunofluorescence: CYP26A1 Antibody [NBP1-52388] - CYP26A1 antibody (2.5µg/ml) staining of PFA-fixed and TX100-treated HeLa cells after primary labelling overnight at 4C with DAPI nuclear counterstaining in blue.

Immunohistochemistry-Paraffin: CYP26A1 Antibody [NBP1-52388] - Analysis of anti-CYP26A1 antibody with human brain, cerebellum, white matter at concentration 3.75 ug/ml.

Immunohistochemistry-Paraffin: CYP26A1 Antibody [NBP1-52388] - Staining of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 3.75 ug/ml.

Product Discontinued

CYP26A1 Antibody Summary

• Immunogen: Peptide with sequence C-NLPARFTHFHGE, from the internal region of the CYP26A1 protein sequence according to NP_000774.2 and NP_476498.1.
• Specificity: Human CYP26A1. This antibody is expected to recognize both reported isoforms (NP_000774.2; NP_476498.1)
• Predicted Species: Primate (92%), Monkey (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: CYP26A1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 2-5 ug/ml
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 3.75 ug/ml
  • Peptide ELISA 1:16000
• Application Notes: IHC: NBP1-52388 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity.

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Gorilla (100%), Gibbon (100%), Marmoset (92%), Rat (83%), Hamster (83%), Bat (83%), Canine (83%), Rabbit (83%).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: TBS (pH 7.3) and 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for CYP26A1 Antibody

• CP26
• CYP26Retinoic acid 4-hydroxylase
• cytochrome P450 26A1
• Cytochrome P450 retinoic acid-inactivating 1
• cytochrome P450, family 26, subfamily A, polypeptide 1
• cytochrome P450, subfamily XXVIA, polypeptide 1
• Cytochrome P450RAI
• EC 1.14
• hP450RAI
• P450, retinoic acid-inactivating, 1
• P450RAI
• P450RAI1Retinoic acid-metabolizing cytochrome

Background

This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This endoplasmic reticulum protein acts on retinoids, including all-trans-retinoic acid (RA), with both 4-hydroxylation and 18-hydroxylation activities. This enzyme regulates the cellular level of retinoic acid which is involved in regulation of gene expression in both embryonic and adult tissues. Two alternatively spliced transcript variants of this gene, which encode the distinct isoforms, have been reported.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CYP26A1
• Entrez:
  • 1592(Human)
• Uniprot:
  • O43174(Human)