Cyclophilin B Antibody (549205) [Unconjugated]

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, A431 human epithelial carcinoma cell line, BaF3 mouse pro-B cell line, PC-12 rat adrenal pheochromocytoma cell line, Rat-2 rat embryonic fibroblast cell line, and NRK rat normal kidney cell. PVDF membrane was probed with 1 µg/mL Mouse Anti-Human/Mouse/ Rat Cyclophilin B Monoclonal Antibody (Catalog # MAB5410) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). For additional reference, recombinant human Cyclophilin A and Cyclophilin B (5 ng/lane) were included. A specific band for Cyclophilin B was detected at approximately 24 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.

Cyclophilin B was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Mouse Anti-Human/Mouse/Rat Cyclophilin B Monoclonal Antibody (Catalog # MAB5410) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and Cyclophilin B knockout HeLa cell line (KO). PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse/Rat Cyclophilin B Monoclonal Antibody (Catalog # MAB5410) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Cyclophilin B at approximately 25 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # MAB5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, KO
• Clone: 549205
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

Cyclophilin B Antibody (549205) [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human Cyclophilin B
  Asp34-Glu216
  Accession # P23284
• Specificity: Detects human, mouse, and rat Cyclophilin B in Western blots. In Western blots, no cross-react with recombinant human Cyclophilin A is observed. Detection of mouse and rat Cyclophilin B has not been tested in Immunocytochemistry.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: PPIB
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 8-25 ug/mL
  • Knockout Validated
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Cyclophilin B Antibody (549205) [Unconjugated]

• Cyclophilin B
• cyclophilin-like protein
• CYPB
• CYPBEC 5.2.1.8
• CYP-S1
• MGC2224
• OI9MGC14109
• peptidyl-prolyl cis-trans isomerase B
• peptidylprolyl isomerase B (cyclophilin B)
• PPIase B
• PPIB
• Rotamase B
• S-cyclophilin
• SCYLP

Background

Cyclophilin B (SCYLP, CyPB and peptidyl-prolyl cis-trans isomerase B) is a 24 kDa glycoprotein member of the B subfamily of the cyclophilin-type PPIase family of molecules. It is both secreted and retained in the ER. When secreted, Cyclophilin B mediates chemotaxis and T cell adhesion to fibronectin. This is likely due to its prolyl cis/trans isomerase activity. Intracellularly, Cyclophilin B appears to serve as a molecular chaperone for molecules destined for secretion. It does so via stabilization, and facilitating the activity of additional chaperones. The human Cyclophilin B precursor is 216 amino acids (aa) in length. It contains a 25 aa signal sequence plus a 191 aa mature region. There is a partial heparin-binding sequence (aa 27‑34), a PPIase domain (aa 47‑204) and a C-terminal ER retention motif (aa 213‑216). Over aa 34‑216, the human and mouse sequences are 95% aa identical.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Cyclophilin B Antibody (MAB5410)(6)

Publications using MAB5410

• David C. Hondius, Frank Koopmans, Conny Leistner, Débora Pita-Illobre, Regina M. Peferoen-Baert, Fenna Marbus et al. The proteome of granulovacuolar degeneration and neurofibrillary tangles in Alzheimer’s disease Acta Neuropathologica 2021-03-01 [PMID: 33492460]
• Ivan Crespo-Enriquez, Tina Hodgson, Sana Zakaria, Erika Cadoni, Mittal Shah, Stephen Allen et al. Dchs1-Fat4 regulation of osteogenic differentiation in mouse Development 2019-07-15 [PMID: 31358536]
• Ivan Crespo-Enriquez, Tina Hodgson, Sana Zakaria, Erika Cadoni, Mittal Shah, Stephen Allen et al. Dchs1-Fat4 regulation of osteogenic differentiation in mouse Development 2019-07-15 [PMID: 31358536] (Western Blot, Mouse)
• Emily J Lodge, Alice Santambrogio, John P Russell, Paraskevi Xekouki, Thomas S Jacques, Randy L Johnson et al. Homeostatic and tumourigenic activity of SOX2+ pituitary stem cells is controlled by the LATS/YAP/TAZ cascade eLife 2019-03-26 [PMID: 30912742]
• Emily J Lodge, Alice Santambrogio, John P Russell, Paraskevi Xekouki, Thomas S Jacques, Randy L Johnson et al. Homeostatic and tumourigenic activity of SOX2+ pituitary stem cells is controlled by the LATS/YAP/TAZ cascade eLife 2019-03-26 [PMID: 30912742] (Western Blot, Mouse)
• Ajay Mishra, Bénédicte Oulès, Angela Oliveira Pisco, Tony Ly, Kifayathullah Liakath-Ali, Gernot Walko et al. A protein phosphatase network controls the temporal and spatial dynamics of differentiation commitment in human epidermis eLife 2017-10-18 [PMID: 29043977]

Bioinformatics

• Gene Symbol: PPIB
• Uniprot:
  • P23284()