Cyclophilin B Antibody (549205) [Unconjugated]

HAF007). For additional reference, recombinant human Cyclophilin A and Cyclophilin B (5 ng/lane) were included. A specific band for Cyclophilin B was detected at approximately 24 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3." > HAF007). For additional reference, recombinant human Cyclophilin A and Cyclophilin B (5 ng/lane) were included. A specific band for Cyclophilin B was detected at approximately 24 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3." title="Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, A431 human epithelial carcinoma cell line, BaF3 mouse pro-B cell line, PC-12 rat adrenal pheochromocytoma cell line, Rat-2 rat embryonic fibroblast cell line, and NRK rat normal kidney cell. PVDF membrane was probed with 1 µg/mL Mouse Anti-Human/Mouse/ Rat Cyclophilin B Monoclonal Antibody (Catalog # MAB5410) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). For additional reference, recombinant human Cyclophilin A and Cyclophilin B (5 ng/lane) were included. A specific band for Cyclophilin B was detected at approximately 24 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3." />

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, A431 human epithelial carcinoma cell line, BaF3 mouse pro-B cell line, PC-12 rat adrenal pheochromocytoma cell line, Rat-2 rat embryonic fibroblast cell line, and NRK rat normal kidney cell. PVDF membrane was probed with 1 µg/mL Mouse Anti-Human/Mouse/ Rat Cyclophilin B Monoclonal Antibody (Catalog # MAB5410) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # <a class=

NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." > NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." title="Cyclophilin B was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Mouse Anti-Human/Mouse/Rat Cyclophilin B Monoclonal Antibody (Catalog # MAB5410) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." />

Cyclophilin B was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Mouse Anti-Human/Mouse/Rat Cyclophilin B Monoclonal Antibody (Catalog # MAB5410) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # <a class=

HAF018). A specific band was detected for Cyclophilin B at approximately 25 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # MAB5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." > HAF018). A specific band was detected for Cyclophilin B at approximately 25 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # MAB5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." title="Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and Cyclophilin B knockout HeLa cell line (KO). PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse/Rat Cyclophilin B Monoclonal Antibody (Catalog # MAB5410) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Cyclophilin B at approximately 25 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # MAB5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." />

Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and Cyclophilin B knockout HeLa cell line (KO). PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse/Rat Cyclophilin B Monoclonal Antibody (Catalog # MAB5410) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # <a class=

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, KO
• Clone: 549205
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

Cyclophilin B Antibody (549205) [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human Cyclophilin B
  Asp34-Glu216
  Accession # P23284
• Specificity: Detects human, mouse, and rat Cyclophilin B in Western blots. In Western blots, no cross-react with recombinant human Cyclophilin A is observed. Detection of mouse and rat Cyclophilin B has not been tested in Immunocytochemistry.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: PPIB
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 8-25 ug/mL
  • Knockout Validated
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Cyclophilin B Antibody (549205) [Unconjugated]

• Cyclophilin B
• cyclophilin-like protein
• CYPB
• CYPBEC 5.2.1.8
• CYP-S1
• MGC2224
• OI9MGC14109
• peptidyl-prolyl cis-trans isomerase B
• peptidylprolyl isomerase B (cyclophilin B)
• PPIase B
• PPIB
• Rotamase B
• S-cyclophilin
• SCYLP

Background

Cyclophilin B (SCYLP, CyPB and peptidyl-prolyl cis-trans isomerase B) is a 24 kDa glycoprotein member of the B subfamily of the cyclophilin-type PPIase family of molecules. It is both secreted and retained in the ER. When secreted, Cyclophilin B mediates chemotaxis and T cell adhesion to fibronectin. This is likely due to its prolyl cis/trans isomerase activity. Intracellularly, Cyclophilin B appears to serve as a molecular chaperone for molecules destined for secretion. It does so via stabilization, and facilitating the activity of additional chaperones. The human Cyclophilin B precursor is 216 amino acids (aa) in length. It contains a 25 aa signal sequence plus a 191 aa mature region. There is a partial heparin-binding sequence (aa 27‑34), a PPIase domain (aa 47‑204) and a C-terminal ER retention motif (aa 213‑216). Over aa 34‑216, the human and mouse sequences are 95% aa identical.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Cyclophilin B Antibody (MAB5410)(7)

Publications using MAB5410

• David C. Hondius, Frank Koopmans, Conny Leistner, Débora Pita-Illobre, Regina M. Peferoen-Baert, Fenna Marbus et al. The proteome of granulovacuolar degeneration and neurofibrillary tangles in Alzheimer’s disease Acta Neuropathologica 2021-03-01 [PMID: 33492460] (Immunohistochemistry, Human)
• David C. Hondius, Frank Koopmans, Conny Leistner, Débora Pita-Illobre, Regina M. Peferoen-Baert, Fenna Marbus et al. The proteome of granulovacuolar degeneration and neurofibrillary tangles in Alzheimer’s disease Acta Neuropathologica 2021-03-01 [PMID: 33492460]
• Ivan Crespo-Enriquez, Tina Hodgson, Sana Zakaria, Erika Cadoni, Mittal Shah, Stephen Allen et al. Dchs1-Fat4 regulation of osteogenic differentiation in mouse Development 2019-07-15 [PMID: 31358536]
• Ivan Crespo-Enriquez, Tina Hodgson, Sana Zakaria, Erika Cadoni, Mittal Shah, Stephen Allen et al. Dchs1-Fat4 regulation of osteogenic differentiation in mouse Development 2019-07-15 [PMID: 31358536] (Western Blot, Mouse)
• Emily J Lodge, Alice Santambrogio, John P Russell, Paraskevi Xekouki, Thomas S Jacques, Randy L Johnson et al. Homeostatic and tumourigenic activity of SOX2+ pituitary stem cells is controlled by the LATS/YAP/TAZ cascade eLife 2019-03-26 [PMID: 30912742]
• Emily J Lodge, Alice Santambrogio, John P Russell, Paraskevi Xekouki, Thomas S Jacques, Randy L Johnson et al. Homeostatic and tumourigenic activity of SOX2+ pituitary stem cells is controlled by the LATS/YAP/TAZ cascade eLife 2019-03-26 [PMID: 30912742] (Western Blot, Mouse)
• Ajay Mishra, Bénédicte Oulès, Angela Oliveira Pisco, Tony Ly, Kifayathullah Liakath-Ali, Gernot Walko et al. A protein phosphatase network controls the temporal and spatial dynamics of differentiation commitment in human epidermis eLife 2017-10-18 [PMID: 29043977]

Bioinformatics

• Gene Symbol: PPIB
• Uniprot:
  • P23284()