Cyclin D3 Antibody

Images

 
Western Blot: Cyclin D3 Antibody [NBP1-31806] - Sample (30 ug of whole cell lysate) A: HepG2 12% SDS PAGE; antibody diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: Cyclin D3 Antibody [NBP1-31806] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Cyclin D3 protein stained by Cyclin D3 antibody diluted at 1:200. Red: ...read more
Immunohistochemistry-Paraffin: Cyclin D3 Antibody [NBP1-31806] - Paraffin-embedded mouse lung. Cyclin D3 antibody diluted at 1:500.
Immunocytochemistry/ Immunofluorescence: Cyclin D3 Antibody [NBP1-31806] - Paraformaldehyde-fixed A431, using antibody at 1:500 dilution.
Immunohistochemistry-Paraffin: Cyclin D3 Antibody [NBP1-31806] - Mouse lung.

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Bv, Mk, ShSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

Cyclin D3 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human Cyclin D3. The exact sequence is proprietary.
Localization
Cytoplasm/Nucleus
Predicted Species
Porcine (97%), Bovine (99%), Sheep (99%), Monkey (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CCND3
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100 - 1:2000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
Theoretical MW
33 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Expected cross reactivity based on sequence homology: Rhesus Monkey (100%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.0), 1.0% BSA and 20% Glycerol
Preservative
0.01% Thimerosal
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Cyclin D3 Antibody

  • CCND3
  • Cyclin D3
  • D3-type cyclin
  • G1/S-specific cyclin D3
  • G1/S-specific cyclin-D3

Background

Cyclin D3 is a member of Cyclin D family which mediates cell cycle progression through the G1 phase. Cyclin D family binds and activates cyclin-dependent kinases 4 and 6 (CDK4, CDK6), which regulates the phosphorylation of retinoblastoma (Rb) protein, p107, and p130 (1). Cyclin D3, similar to cyclin D1 and D2, regulates the initial G1 to S transition by inhibiting Rb and activating E2F proteins (2). Cyclin D3 expression has been linked to inhibition of granulocyte differentiation and activation of various transcription factors, such as transcription factor 5 (3, 4). Overexpression of cyclin D3 is associated with poor clinical outcome in diffuse large B-cell lymphoma cases (5).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol CCND3
Entrez