Cyclin D3 Antibody (SD20-43)

Western Blot: Cyclin D3 Antibody (SD20-43) [NBP2-67769] - Analysis of Cyclin D3 on different lysates using anti-Cyclin D3 antibody at 1/1,000 dilution. Positive control: Lane 1: Jurkat Lane 2: Hela

Immunocytochemistry/Immunofluorescence: Cyclin D3 Antibody (SD20-43) [NBP2-67769] - Staining Cyclin D3 in PANC-1 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Flow Cytometry: Cyclin D3 Antibody (SD20-43) [NBP2-67769] - Analysis of Hela cells with Cyclin D3 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.

Immunocytochemistry/Immunofluorescence: Cyclin D3 Antibody (SD20-43) [NBP2-67769] - Staining Cyclin D3 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunocytochemistry/Immunofluorescence: Cyclin D3 Antibody (SD20-43) [NBP2-67769] - Staining Cyclin D3 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Product Discontinued

Cyclin D3 Antibody (SD20-43) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Recombinant protein within Human Cyclin D3 aa 162-292 / 292. (SwissProt: P30281 Human; SwissProt: P30282 Mouse)
• Localization: Nucleus, Cytoplasm, Membrane.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: CCND3
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:10-1:50
  • Immunocytochemistry/ Immunofluorescence 1:50-1:100
  • Western Blot 1:500-1:1000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for Cyclin D3 Antibody (SD20-43)

• CCND3
• Cyclin D3
• D3-type cyclin
• G1/S-specific cyclin D3
• G1/S-specific cyclin-D3

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CCND3