Cyclin D3 Antibody (DCS-22)

Product Discontinued

Cyclin D3 Antibody (DCS-22) Summary

• Immunogen: Recombinant human cyclin D3
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: CCND3
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 1:20-1:40
  • Western Blot 1:100-1:2000
• Application Notes: This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. 0.01M Sodium citrate buffer pH6.0 is recommended for this purpose.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 1 ml S/N with 0.9% Sodium Azide
• Preservative: 0.9% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein A or G purified

Alternate Names for Cyclin D3 Antibody (DCS-22)

• CCND3
• Cyclin D3
• D3-type cyclin
• G1/S-specific cyclin D3
• G1/S-specific cyclin-D3

Background

Cyclin D3 is a member of Cyclin D family which mediates cell cycle progression through the G1 phase. Cyclin D family binds and activates cyclin-dependent kinases 4 and 6 (CDK4, CDK6), which regulates the phosphorylation of retinoblastoma (Rb) protein, p107, and p130 (1). Cyclin D3, similar to cyclin D1 and D2, regulates the initial G1 to S transition by inhibiting Rb and activating E2F proteins (2). Cyclin D3 expression has been linked to inhibition of granulocyte differentiation and activation of various transcription factors, such as transcription factor 5 (3, 4). Overexpression of cyclin D3 is associated with poor clinical outcome in diffuse large B-cell lymphoma cases (5).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Cyclin D3 Antibody (NBP1-51744)(1)

Publication using NBP1-51744

• Bartkova J, Zemanova M, Bartek J et al. Abundance and subcellular localisation of cyclin D3 in human tumours. Int J Cancer. 1996-01-01 [PMID: 8575852]

Bioinformatics

• Gene Symbol: CCND3
• Entrez:
  • 896(Human)
• Uniprot:
  • P30281()