Cullin 2 Antibody

Product Discontinued

Cullin 2 Antibody Summary

• Immunogen: The antiserum was produced against synthesized peptide derived from human Cullin 2. The immunogen region is: C-terminal.
• Specificity: Cullin 2 Antibody detects endogenous levels of total Cullin 2 protein.
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CUL2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:5000
  • Immunocytochemistry/ Immunofluorescence 1:100-1:500
  • Immunohistochemistry 1:50-1:100
  • Immunohistochemistry-Paraffin 1:50-1:100
  • Western Blot 1:500-1:1000
• Theoretical MW: 86 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (without Mg2+ and Ca2+, pH 7.4), 0.15M NaCl and 50% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Cullin 2 Antibody

• CUL2
• CUL-2
• Cullin 2
• cullin-2
• MGC131970

Background

Cullin proteins comprise a distinct family of mediators that participate in the selective targeting of proteins for ubiquitin (Ub)-mediated proteolysis. CUL-1, which is the mammalian homolog of Cdc53 from yeast, is an integral component of the E3 ubiquitin ligase complex designated SCF. The SCF (Skp1/CUL-1/F-box protein complex) consists of Skp1 associating with both CUL-1 and an F-box protein, such as Skp2, which determines the substrate specificity of the complex. CUL-1 mediated ubiquitination results in the degradation of cell cycle proteins cyclin D, p21 and cyclin E. Another cullin, CUL-3 facilitates the degradation of cyclin E independent of SCF activity, while CUL-2 associates with the tumor suppressing protein VHL and elongin B to form VBC complexes, which structurally resemble the SCF ligase. Proteolysis also occurs by way of CUL-4 associating with Nedd-8, a ubiquitin- like protein, where it too functions as an active component of a multifunctional E3 complex. CUL-5, or vasopressin-activated, calcium-mobilizing protein (VACM-1), is also included in the cullin family as it shares substantial sequence homology with CUL-1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Cullin 2 Antibody (NBP1-67535)(2)

Publications using NBP1-67535

• Vava A, Paccez JD, Wang Y et al. DCUN1D1 Is an Essential Regulator of Prostate Cancer Proliferation and Tumour Growth That Acts through Neddylation of Cullin 1, 3, 4A and 5 and Deregulation of Wnt/Catenin Pathway Cells 2023-07-31 [PMID: 37566052] (Western Blot, Human)
• Cukras S, Morffy N, Ohn T, Kee Y. Inactivating UBE2M Impacts the DNA Damage Response and Genome Integrity Involving Multiple Cullin Ligases. PLoS ONE 2014-07-16 [PMID: 25025768] (WB, Human)
  
  Details:
  a-CUL2 /Cullin 2 antibody used for WB on HEY ovarian cancer cells treated or not with siRNA for depletion/knock-down of CUL2 (Figure 3A, Figure 4C, Figure S7) or UBE2M (Figure S6).

Bioinformatics

• Gene Symbol: CUL2
• Uniprot:
  • Q13617(Human)