CUGBP1/CELF1 Knockout 293T Cell Lysate

Product Discontinued

CUGBP1/CELF1 Knockout 293T Cell Lysate Summary

• Preparation Method: Knockout achieved by using CRISPR/Cas9,5 bp deletion in exon 2 and 4 bp deletion in exon 2
• Gene: CELF1

Applications/Dilutions

• Dilutions:
  • Western Blot
• Application Notes: You will receive 1 vial (100ug) of knockout cell lysate and 1 vial (100ug) of Parental cell lysate. Lysate can be diluted with 1X SDS sample buffer and will be stable at -20 degrees C for 12 months. Minimize freeze-thaw cycles.

Packaging, Storage & Formulations

• Storage: Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.
• Buffer: 0.1 mg cell homogenate lyophilized in RIPA buffer made with double-knockout cell lines.
• Concentration: LYOPH
• Reconstitution Instructions: To use as WB negative control, spin down briefly and resuspend in 100 uL 1xSDS sample buffer (2% SDS, 60 mM Tris-HCl pH 6.8, 10% Glycerol, 0.02% Bromophenol blue, 60 mM beta-mercaptoethanol). Boil the lysate for 3 - 5 minutes before loading it onto gel.

Lysate Details for Array

• Type: Knockout 293T Cell
• Life Stage: Embryonic
• Tissue: Kidney

Notes

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Validation of antibody specificity is critical and verification of antibody performance against knockout samples is one way to guarantee that an antibody recognizes a specific target. Novus' KO cell lysate can be used as a negative control for western blots and to confirm the specificity of antibodies.

Alternate Names for CUGBP1/CELF1 Knockout 293T Cell Lysate

• BRUNOL2
• BRUNOL250 kDa nuclear polyadenylated RNA-binding protein
• bruno-like 2
• Bruno-like protein 2
• CELF1
• CUG RNA-binding protein
• CUG triplet repeat, RNA binding protein 1
• CUG triplet repeat, RNA-binding protein 1
• CUG-BP- and ETR-3-like factor 1
• CUGBP Elav-like family member 1
• CUG-BP
• CUGBP, Elav-like family member 1
• CUGBP1
• CUG-BP1
• CUGBP1CUG triplet repeat RNA-binding protein 1
• CUGBPCELF-1
• D2Wsu101e
• Deadenylation factor CUG-BP
• EDEN-BP homolog
• EDEN-BP
• embryo deadenylation element binding protein
• Embryo deadenylation element-binding protein homolog
• HNAB50
• NAB50
• NAB50CUG-BP1
• NAPOR
• nuclear polyadenylated RNA-binding protein, 50-kD
• RNA-binding protein BRUNOL-2

Background

Members of the CELF/BRUNOL protein family contain two N-terminal RNA recognition motif (RRM) domains, one C-terminal RRM domain, and a divergent segment of 160-230 aa between the second and third RRM domains. Members of this protein family regulate pre-mRNA alternative splicing and may also be involved in mRNA editing, and translation. This gene may play a role in myotonic dystrophy type 1 (DM1) via interactions with the dystrophia myotonica-protein kinase (DMPK) gene. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Bioinformatics

• Gene Symbol: CELF1