CUGBP1/CELF1 Overexpression Lysate (Native)


Western Blot: CUGBP1 Overexpression Lysate (Adult Normal) [NBL1-09605] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for CUGBP1.

Product Details

Reactivity HuSpecies Glossary
Applications WB

Order Details

CUGBP1/CELF1 Overexpression Lysate (Native) Summary

The lysate was created in HEK293T cells, using plasmid ID RC221943 and based on accession number NM_198700. The protein contains a C-terminal DDK tag.
Homo sapiens CUG triplet repeat, RNA binding protein 1 (CUGBP1), transcript variant 2, mRNA.


Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
51.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Store at -80C. Avoid freeze-thaw cycles.
RIPA buffer

Lysate Details for CUGBP1/CELF1

Protein State


HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for CUGBP1/CELF1 Overexpression Lysate (Native)

  • BRUNOL250 kDa nuclear polyadenylated RNA-binding protein
  • bruno-like 2
  • Bruno-like protein 2
  • CELF1
  • CUG RNA-binding protein
  • CUG triplet repeat, RNA binding protein 1
  • CUG triplet repeat, RNA-binding protein 1
  • CUG-BP- and ETR-3-like factor 1
  • CUGBP Elav-like family member 1
  • CUG-BP
  • CUGBP, Elav-like family member 1
  • CUGBP1
  • CUG-BP1
  • CUGBP1CUG triplet repeat RNA-binding protein 1
  • D2Wsu101e
  • Deadenylation factor CUG-BP
  • EDEN-BP homolog
  • embryo deadenylation element binding protein
  • Embryo deadenylation element-binding protein homolog
  • HNAB50
  • NAB50
  • NAB50CUG-BP1
  • nuclear polyadenylated RNA-binding protein, 50-kD
  • RNA-binding protein BRUNOL-2


Members of the CELF/BRUNOL protein family contain two N-terminal RNA recognition motif (RRM) domains, one C-terminal RRM domain, and a divergent segment of 160-230 aa between the second and third RRM domains. Members of this protein family regulate pre-mRNA alternative splicing and may also be involved in mRNA editing, and translation. This gene may play a role in myotonic dystrophy type 1 (DM1) via interactions with the dystrophia myotonica-protein kinase (DMPK) gene. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq]. Transcript Variant: This variant (2) uses a different segment for its 5' UTR and a different internal splice site in its coding region, compared to variant 1. The resulting isoform (2) has an additional internal amino acid compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

Species: Hu
Applications: WB
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
Species: Hu, Mu
Applications: WB, ELISA, IHC, IP
Species: Hu
Applications: WB, ELISA, Flow, ICC/IF
Species: Hu
Applications: ICC/IF, IHC, IHC-P
Species: Hu, Mu, Rt, Pm
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
Species: Hu, Mu
Applications: WB, IP
Species: Hu
Applications: ICC/IF, IHC, IHC-P
Species: Hu
Applications: WB, ELISA, IHC, IHC-P
Species: Hu, Mu, Rt, Ca
Applications: IA, S-ELISA
Species: Hu
Applications: WB, IHC
Species: Hu, Mu, Rt, Bv
Applications: WB, ICC/IF, IHC, IHC-P
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
Species: Hu, Ca, Mk
Applications: WB, IHC, IHC-P
Species: Hu, Mu, Rt, Bv, Ha, Pm
Applications: WB, Simple Western, B/N, DB, EM, ICC/IF, IHC, IHC-P, IP, PA
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
Species: Hu
Applications: WB, IHC, IHC-P, IP
Species: Hu
Applications: WB

Publications for CUGBP1/CELF1 Lysate (NBL1-09605) (0)

There are no publications for CUGBP1/CELF1 Lysate (NBL1-09605).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for CUGBP1/CELF1 Lysate (NBL1-09605) (0)

There are no reviews for CUGBP1/CELF1 Lysate (NBL1-09605). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for CUGBP1/CELF1 Lysate (NBL1-09605) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional CUGBP1/CELF1 Products

Bioinformatics Tool for CUGBP1/CELF1 Lysate (NBL1-09605)

Discover related pathways, diseases and genes to CUGBP1/CELF1 Lysate (NBL1-09605). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for CUGBP1/CELF1 Lysate (NBL1-09605)

Discover more about diseases related to CUGBP1/CELF1 Lysate (NBL1-09605).

Pathways for CUGBP1/CELF1 Lysate (NBL1-09605)

View related products by pathway.

PTMs for CUGBP1/CELF1 Lysate (NBL1-09605)

Learn more about PTMs related to CUGBP1/CELF1 Lysate (NBL1-09605).

Research Areas for CUGBP1/CELF1 Lysate (NBL1-09605)

Find related products by research area.

Blogs on CUGBP1/CELF1

There are no specific blogs for CUGBP1/CELF1, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs


Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.


Review this Product

Be the first to review our CUGBP1/CELF1 Overexpression Lysate (Native) and receive a gift card or discount.


Gene Symbol CELF1