CRABP2 Antibody

Western blot shows lysates of MCF-7 human breast cancer cell line, MDA-MB-468 human breast cancer cell line, and T47D human breast cancer cell line. PVDF membrane was probed with 0.5 µg/mL of Human CRABP2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5740) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for CRABP2 at approximately 14 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Hu
• Applications: WB
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

CRABP2 Antibody Summary

• Immunogen: E. coli-derived recombinant human CRABP2
  Met1-Glu138
  Accession # P29373
• Specificity: Detects endogenous human CRABP2 in Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: CRABP2
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 0.5 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CRABP2 Antibody

• cellular retinoic acid binding protein 2
• Cellular retinoic acid-binding protein II
• CRABP2
• CRABP-II
• CRABP-IIcellular retinoic acid-binding protein 2
• RBP6

Background

CRABP2 (Cellular retinoic acid binding protein type II; also RABP2) is a 14 kDa cytosolic member of the fatty acid binding protein family of molecules. It is expressed by a variety of cell types, including keratinocytes, corneal epithelium, fibroblasts and visceral smooth muscle. CRABP2 binds bioactive retinoic acid and transports it to the nucleus, where, in contrast to CRABP1, it facilitates its transfer to the retinoic acid receptor. Human CRABP2 is 138 amino acids (aa) in length and contains one NLS (aa 21‑31) and a series of beta -strands forming a beta -barrel that accommodates retinoic acid in its interior (aa 41‑135).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CRABP2 Antibody (AF5740)(1)

Publication using AF5740

• P Thulasiram, G Garriga, V Danthuluri, DJ McAndrews, IQ Mohiuddin Activation of the CRABPII/RAR pathway by curcumin induces retinoic acid mediated apoptosis in retinoic acid resistant breast cancer cells Oncol. Rep, 2017-03-08;0(0):. 2017-03-08 [PMID: 28350049] (Western Blot, Human)

Bioinformatics

• Gene Symbol: CRABP2
• Uniprot:
  • P29373()