Coronin-1a Antibody

Western Blot: Coronin-1a Antibody [NB100-1298] - Staining of Human Lymph node (A) and Spleen (B) lysate (35 ug protein in RIPA buffer). Antibody at 0.5 ug/mL. Detected by chemiluminescence.

Immunohistochemistry-Paraffin: Coronin-1a Antibody [NB100-1298] - Staining of paraffin embedded human spleen. Antibody at 3.75 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Flow Cytometry: Coronin-1a Antibody [NB100-1298] - Flow cytometric analysis of paraformaldehyde fixed Jurkat cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Western Blot: Coronin-1a Antibody [NB100-1298] - Staining of Daudi (A), MOLT4 (B) and (1ug/ml) of Jurkat (C) cell lysate (35 ug protein in RIPA buffer). Antibody at 0.5 ug/mL. Detected by chemiluminescence.

Immunohistochemistry-Paraffin: Coronin-1a Antibody [NB100-1298] - Staining of paraffin embedded human small Intestine. Antibody at 3.75 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

• Reactivity: Hu, Mu, Rt, Bv, Ca
• Applications: WB, ELISA, Flow, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

Coronin-1a Antibody Summary

• Description: Novus Biologicals Goat Coronin-1a Antibody (NB100-1298) is a polyclonal antibody validated for use in IHC, WB, ELISA and Flow. Anti-Coronin-1a Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-KRLDRLEETVQAK corresponding to C-Terminus according to NP_009005.1.
• Localization: In noninfected macrophages, associated with the cortical microtubule network; in mycobacteria-infected macrophages, becomes progressively relocalized and retained around the mycobacterial phagosomes. Retention on the phagosomal membrane is strictly depend
• Predicted Species: Mouse (100%), Rat (100%), Canine (100%), Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: CORO1A
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 ug/mL
  • Immunohistochemistry 3.75 ug/mL
  • Immunohistochemistry-Paraffin 3.75 ug/mL
  • Peptide ELISA Detection limit 1:16000
  • Western Blot 0.3 - 1 ug/mL
• Control:

  Coronin-1a Overexpression Lysate

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Rat, Canine, Bovine, Mouse.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Coronin-1a Antibody

• CLIPINA
• clipin-A
• CORO1
• coronin, actin binding protein, 1A
• coronin, actin-binding protein, 1A
• coronin-1
• coronin-1A
• Coronin-like protein A
• Coronin-like protein p57
• FLJ41407
• HCORO1
• MGC117380
• p57
• TACOCLABP
• Tryptophan aspartate-containing coat protein

Background

Coronins are actin-binding proteins in eucaryotic cells. The prototype of coronin were found in Dictyostelium discoideum. The protein is involved in many cell processes, e.g. phagocytosis, cytokinesis and migration.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Coronin-1a Antibody (NB100-1298)(1)

Publication using NB100-1298

• Ferrari G, Langen H, Naito M, Pieters J. A coat protein on phagosomes involved in the intracellular survival of mycobacteria. Cell 1999-05-14 [PMID: 10338208]

Bioinformatics

• Gene Symbol: CORO1A
• Entrez:
  • 11151(Human)
  • 12721(Mouse)
  • 155151(Rat)
• Uniprot:
  • NP_009005.(Human)