COPS8 Overexpression Lysate (Native)

Images

 
Western Blot: COPS8 Overexpression Lysate (Adult Normal) [NBL1-09386] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for COPS8.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

COPS8 Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC207324 and based on accession number NM_006710. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens COP9 constitutive photomorphogenic homolog subunit 8 (Arabidopsis) (COPS8), transcript variant 1, mRNA.
Gene
COPS8

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
23 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for COPS8

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for COPS8 Overexpression Lysate (Native)

  • COP9 constitutive photomorphogenic homolog subunit 8 (Arabidopsis)
  • COP9 homolog
  • COP9 signalosome complex subunit 8
  • COP9
  • CSN8JAB1-containing signalosome subunit 8
  • hCOP9
  • MGC1297
  • MMMEP
  • SGN8MGC43256
  • Signalosome subunit 8

Background

The protein encoded by this gene is one of the eight subunits of COP9 signalosome, a highly conserved protein complex that functions as an important regulator in multiple signaling pathways. The structure and function of COP9 signalosome is similar to that of the 19S regulatory particle of 26S proteasome. COP9 signalosome has been shown to interact with SCF-type E3 ubiquitin ligases and act as a positive regulator of E3 ubiquitin ligases. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq]. Transcript Variant: This variant (1) encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Publications for COPS8 Lysate (NBL1-09386) (0)

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Bioinformatics

Gene Symbol COPS8