Connexin 50/GJA8 Antibody

Product Discontinued

Connexin 50/GJA8 Antibody Summary

• Immunogen: Peptide with sequence TPSLCPELTTDDAR corresponding to internal region according to NP_005258.2.
• Epitope: TPSLCPELTTDDAR
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: GJA8
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:4000
• Application Notes: WB: Preliminary experiments in human brain (spinal cord), mouse and rat eye lysates gave no specific signal but low background.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Notes

Please note, this antibody is considered Innovators Grade. Innovators Grade antibodies are generally unvalidated and require additional characterization for most new species/applications. Novus has made these antibodies available through our Innovators Reward program. Complete an online review with image, detailing your positive or negative results. In return, you receive a discount voucher for 100% of the purchase price of the reviewed product. Please contact us at innovators@novusbio.com for more details.

Alternate Names for Connexin 50/GJA8 Antibody

• alpha 8, 50kD (connexin 50)
• CAE
• CAE1
• Connexin 50
• Connexin-50
• CX50
• CZP1
• gap junction alpha-8 protein
• gap junction membrane channel protein alpha-8
• gap junction protein, alpha 8, 50kDa (connexin 50)
• gap junction protein, alpha 8, 50kDa
• GJA8
• lens intrinsic membrane protein MP70
• MP70

Background

The 433 amino acid long, 48 kDA transmembrane connexin, gap junction alpha-8 protein encoded by the GJA8 gene is critical in growth and development of lens fiber cells. Mutations in the GJA8 gene may lead to zonular pulverulent cataracts, nuclear progressive cataracts, and cataract-microcornea syndrome. The GJA8 gene has been linked to myopia, microphthalmia, neuronitis, cycstic fibrosis, neuopathy, cervical adenocarcinoma, and juvenile myoclonic epilepsy. The GJA8 gene interacts with TJP1, MIP, MED14, GJA1, and GJB1 to participate in pathways such as gap junction assembly, membrane trafficking, and connexin synthesis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Connexin 50/GJA8 Antibody (NBP1-52143)(1)

Publication using NBP1-52143

• Lin Y, Liu NN, Lei CT et al. A novel GJA8 mutation in a Chinese family with autosomal dominant congenital cataract. Zhonghua Yi Xue Yi Chuan Xue Za Zhi 2008-02-01 [PMID: 18247306]

Bioinformatics

• Gene Symbol: GJA8
• Entrez:
  • 2703(Human)
  • 14616(Mouse)
  • 29601(Rat)
• Uniprot:
  • NP_005258.2(Human)