Connexin 31/GJB3 Overexpression Lysate (Native)

Images

 
Western Blot: GJB3 Overexpression Lysate (Adult Normal) [NBL1-11096] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for GJB3.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

Connexin 31/GJB3 Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC204405 and based on accession number NM_001005752. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens gap junction protein, beta 3, 31kDa (GJB3), transcript variant 2, mRNA.
Gene
GJB3

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
30.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Connexin 31/GJB3

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for Connexin 31/GJB3 Overexpression Lysate (Native)

  • Connexin 31
  • connexin-31
  • Cx31
  • CX31MGC102938
  • DFNA2
  • DFNA2B
  • EKV
  • erythrokeratodermia variabilis
  • FLJ22486
  • gap junction beta-3 protein
  • gap junction protein, beta 3, 31kD (connexin 31)
  • gap junction protein, beta 3, 31kDa (connexin 31)
  • gap junction protein, beta 3, 31kDa
  • GJB3

Background

This gene is a member of the connexin gene family. The encoded protein is a component of gap junctions, which are composed of arrays of intercellular channels that provide a route for the diffusion of low molecular weight materials from cell to cell. Mutations in this gene can cause non-syndromic deafness or erythrokeratodermia variabilis, a skin disorder. Alternative splicing results in multiple transcript variants encoding the same protein. [provided by RefSeq]. Transcript Variant: This variant (2) differs in the 5' UTR, compared to variant 1. Variants 1 and 2 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Publications for Connexin 31/GJB3 Lysate (NBL1-11096) (0)

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Bioinformatics

Gene Symbol GJB3
Entrez