Complement Component C3aR Antibody (620613) [Unconjugated]

HEK293 human embryonic kidney cell line transfected with mouse Complement Component C3aR and eGFP was stained with (A) Rat Anti-Mouse Complement Component C3aR Monoclonal Antibody (Catalog # MAB10417) or (B) Rat IgG2a isotype control antibody (Catalog # MAB006) followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Mu
• Applications: Flow, CyTOF-ready
• Clone: 620613
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated

Complement Component C3aR Antibody (620613) [Unconjugated] Summary

• Immunogen: Chinese hamster ovary cell line CHO-derived transfected with mouse C3aR
  Accession # O09047
• Specificity: Detects mouse C3aR in direct ELISAs.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Complement Component C3aR Antibody (620613) [Unconjugated]

• AZ3B
• AZ3BC3a anaphylatoxin chemotactic receptor
• C3AR
• C3a-R
• C3AR1
• C3ARcomplement component 3 receptor 1
• C3R1
• complement component 3a receptor 1
• Complement Component C3a R
• Complement Component C3aR
• HNFAG09

Background

Complement Component 3a receptor (C3a R) is an ~90 kDa G-protein coupled 7-transmembrane sulfated glycoprotein expressed on myeloid peripheral blood leukocytes, and on activated lymphocytes, endothelial and internal organ epithelial cells. Engagement of C3a R by C3a activates mast cells and neutrophils, triggering degranulation in airways during asthmatic allergen challenges. It assists in Th2-type inflammatory reactions and stimulates leukocyte chemotaxis. Human C3a R extracellular regions share 50% and 42% amino acid identity with mouse and rat C3a R, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • O09047()