Complement Component C3aR Antibody (14D4)

• Reactivity: Mu
• Applications: Flow, IHC
• Clone: 14D4
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Concentration: 0.1 mg/ml

Complement Component C3aR Antibody (14D4) Summary

• Description: Novus Biologicals Rat Complement Component C3aR Antibody (14D4) (NBP3-11202) is a monoclonal antibody validated for use in IHC and Flow. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: RBL-2H3 transfectants expressing mouse C3aR
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: C3AR1
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry
  • Immunohistochemistry-Frozen
• Application Notes: For immunohistochemistry and Flow Cytometry, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: 0.2 um filtered solution in PBS, 0.1% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.1 mg/ml
• Purity: Protein G purified

Alternate Names for Complement Component C3aR Antibody (14D4)

• AZ3B
• AZ3BC3a anaphylatoxin chemotactic receptor
• C3AR
• C3a-R
• C3AR1
• C3ARcomplement component 3 receptor 1
• C3R1
• complement component 3a receptor 1
• Complement Component C3a R
• Complement Component C3aR
• HNFAG09

Background

The monoclonal antibody 14D4 reacts with mouse C3aR, which is a member of the rhodopsin superfamily of 7-transmembrane G protein-coupled receptors, with a molecular weight of approximately 54 kDa. Expression of C3aR has been demonstrated on a wide variety of immune cells, including monocytes, macrophages, dendritic cells, neutrophils, basophils, eosinophils, mast cells, T lymphocytes and B lymphocytes. In addition, C3aR is found on cells of the central nervous system, lungs and kidney. In the course of complement activation C3aR functions as the cell surface receptor for C3a, which is the C-terminal 77 amino acid cleavage product of C3. C3a, possesses both anaphylatoxic and immunoregulatory properties, such as smooth muscle contraction, histamine release from mast cells, and enhanced vascular permeability. In addition, C3a induces respiratory burst in neutrophils and has chemotactic properties for eosinophils and mast cells. Moreover, C3a causes release of key cytokines from multiple cell types, including IL-1 beta, TNF-alpha, IL-6 and IL-8. A role for C3aR has been implicated in several murine disease models. It was shown that C3aR inhibition reduces neurodegeneration in experimental lupus, whereas in a murine model of allergic airway disease, deletion of the C3a receptor protects against the changes in lung physiology seen after allergen challenge. Finally, it was shown that deletion of C3aR is protective in myelin oligodendrocyte glycoprotein-induced experimental autoimmune encephalomyelitis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: C3AR1